Molecular diagnosis of Anaplasmataceae organisms in dogs with clinical and microscopical signs of ehrlichiosis
Ehrlichioses are important emerging zoonotic tick-borne diseases that can affect both animals and humans. Clinical manifestations of ehrlichiosis caused by different members of Anaplasmataceae in dogs are similar to each other and to other diseases showing systemic manifestation. The observation of...
Saved in:
| Published in: | Revista brasileira de parasitologia veterinaria Vol. 18; no. 4; pp. 20 - 25 |
|---|---|
| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Brazil
Colégio Brasileiro de Parasitologia Veterinária
01-10-2009
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Ehrlichioses are important emerging zoonotic tick-borne diseases that can affect both animals and humans. Clinical manifestations of ehrlichiosis caused by different members of Anaplasmataceae in dogs are similar to each other and to other diseases showing systemic manifestation. The observation of inclusions in white blood cells and in platelets cannot be used to confirm the Anaplasmataceae etiologic agent of the disease. In this work we assessed the presence of Anaplasmataceae agents in 51 dogs from two different cities (Jaboticabal and Campo Grande) showing clinical and microscopical diagnosis of ehrlichiosis, by using molecular techniques. Anaplasmataceae DNA were amplified in 46/51 (90.2%) of the blood samples; 22 (40%) samples from Jaboticabal and 10 (18.2%) from Campo Grande were positive for E. canis nPCR. Anaplasma platys DNA was amplified in 2 samples from Jaboticabal and in 11 from Campo Grande. Phylogenetic analysis of E. canis and A. platys DNA confirmed the infection agent and showed that PCR is the most reliable method to diagnose ehrlichial infection. |
|---|---|
| AbstractList | Ehrlichioses are important emerging zoonotic tick-borne diseases that can affect both animals and humans. Clinical manifestations of ehrlichiosis caused by different members of Anaplasmataceae in dogs are similar to each other and to other diseases showing systemic manifestation. The observation of inclusions in white blood cells and in platelets cannot be used to confirm the Anaplasmataceae etiologic agent of the disease. In this work we assessed the presence of Anaplasmataceae agents in 51 dogs from two different cities (Jaboticabal and Campo Grande) showing clinical and microscopical diagnosis of ehrlichiosis, by using molecular techniques. Anaplasmataceae DNA were amplified in 46/51 (90.2%) of the blood samples; 22 (40%) samples from Jaboticabal and 10 (18.2%) from Campo Grande were positive for E. canis nPCR. Anaplasma platys DNA was amplified in 2 samples from Jaboticabal and in 11 from Campo Grande. Phylogenetic analysis of E. canis and A. platys DNA confirmed the infection agent and showed that PCR is the most reliable method to diagnose ehrlichial infection. |
| Author | Dagnone, Ana Sílvia Machado, Rosangela Zacarias Souza, Alda Izabel de André, Marcos Rogério |
| AuthorAffiliation | Universidade Estadual Paulista Universidade para o Desenvolvimento do Estado e da Região do Pantanal |
| AuthorAffiliation_xml | – name: Universidade para o Desenvolvimento do Estado e da Região do Pantanal – name: Universidade Estadual Paulista |
| Author_xml | – sequence: 1 givenname: Ana Sílvia surname: Dagnone fullname: Dagnone, Ana Sílvia organization: Departamento de Patologia Veterinária, Universidade Estadual Paulista, Brazil – sequence: 2 givenname: Alda Izabel de surname: Souza fullname: Souza, Alda Izabel de – sequence: 3 givenname: Marcos Rogério surname: André fullname: André, Marcos Rogério – sequence: 4 givenname: Rosangela Zacarias surname: Machado fullname: Machado, Rosangela Zacarias |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/20040204$$D View this record in MEDLINE/PubMed |
| BookMark | eNo9kc1v1DAQxS1URLeFI1fkG6csY4_j2Meq4ksq4gBI3CzHcXZdOXawNyD-e5Juy2VGGv3mjea9K3KRcvKEvGawF8j5u9LPv_fAFAgA8YzsmFai4VqyC7IDBtgoIX9ekqta7wEkAOALcslXFjiIHUlfcvRuibbQIdhDyjVUmkd6k-wcbZ3syTpvPc3lYFOoU6Uh0SEfKv0TTkfqYkjB2UhtGugUXMnV5flhUsMhPUj5Y4nBHcMm_ZI8H22s_tVjvyY_Prz_fvupufv68fPtzV3jEODUYN-qTmps27XjaJXgvrNy1CPiKHnb92KwcmBSad5ZjRqVZKLzIDVnGgVek_1Zt7rgYzb3eSlpPWi-bf6YzZ_VAw2bD1tZF96eF-aSfy2-nswUqvMx2uTzUk2HKFFhq1ayOZPbs7X40cwlTLb8NQzMFonZIjFPkaz8m0flpZ_88J9-ygD_AUZWhu8 |
| CitedBy_id | crossref_primary_10_1590_S1984_29612012005000009 crossref_primary_10_1590_S1984_29612014071 crossref_primary_10_1016_j_ttbdis_2020_101517 crossref_primary_10_1590_s0100_736x2017000200006 crossref_primary_10_1590_0103_8478cr20150090 crossref_primary_10_1590_s1984_29612016066 crossref_primary_10_1590_0001_3765201720150556 crossref_primary_10_1590_S1984_29612011000100002 crossref_primary_10_1590_s1984_296120180068 crossref_primary_10_1590_s1984_296120180069 crossref_primary_10_1016_j_prevetmed_2012_10_011 crossref_primary_10_1016_j_ttbdis_2015_07_004 crossref_primary_10_1590_S1984_29612013000300007 crossref_primary_10_1016_j_cimid_2012_02_001 crossref_primary_10_1590_S1984_29612013000400012 crossref_primary_10_17221_7380_VETMED crossref_primary_10_1100_2012_605743 crossref_primary_10_1590_S0103_84782012000800021 crossref_primary_10_1590_S1984_29612015030 crossref_primary_10_1016_j_ttbdis_2014_03_011 crossref_primary_10_1016_j_vprsr_2021_100539 crossref_primary_10_1016_j_cimid_2018_11_015 crossref_primary_10_1016_j_vetpar_2017_11_005 crossref_primary_10_1590_S1984_29612013000100019 crossref_primary_10_1089_vbz_2013_1303 crossref_primary_10_1590_S1984_29612015019 crossref_primary_10_1177_1040638711406974 crossref_primary_10_1016_j_vetpar_2011_11_011 crossref_primary_10_1016_j_cimid_2020_101563 crossref_primary_10_1016_j_ttbdis_2012_04_002 |
| ContentType | Journal Article |
| Copyright | This work is licensed under a Creative Commons Attribution 4.0 International License. |
| Copyright_xml | – notice: This work is licensed under a Creative Commons Attribution 4.0 International License. |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 GPN |
| DOI | 10.4322/rbpv.01804004 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic SciELO |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
| DatabaseTitleList | MEDLINE |
| Database_xml | – sequence: 1 dbid: ECM name: MEDLINE url: https://search.ebscohost.com/login.aspx?direct=true&db=cmedm&site=ehost-live sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Veterinary Medicine |
| DocumentTitleAlternate | Diagnóstico molecular de agentes da família Anaplasmataceae em cães com sinais clínicos e microscópios de erliquiose |
| EISSN | 1984-2961 |
| EndPage | 25 |
| ExternalDocumentID | S1984_29612009000400004 10_4322_rbpv_01804004 20040204 |
| Genre | Journal Article |
| GroupedDBID | 123 53G 5VS ABXHO AENEX ALMA_UNASSIGNED_HOLDINGS AZFZN CGR CUY CVF DIK ECM EIF FRP GROUPED_DOAJ IPNFZ KQ8 M~E N.T NPM OK1 PGMZT RIG RNS RPM RSC SCD AAYXX CITATION 7X8 GPN |
| ID | FETCH-LOGICAL-c300t-3b587693555873fa842e7a6f9f33f625bb4da6d168927a939386147e069219343 |
| ISSN | 0103-846X 1984-2961 |
| IngestDate | Tue Nov 19 21:56:29 EST 2024 Sat Aug 17 00:50:12 EDT 2024 Fri Aug 23 02:35:41 EDT 2024 Sat Sep 28 07:45:37 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Keywords | Brasil Dogs Brazil Anaplasma platys Ehrlichia canis PCR Cães |
| Language | English |
| License | This work is licensed under a Creative Commons Attribution 4.0 International License. http://creativecommons.org/licenses/by/4.0 |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c300t-3b587693555873fa842e7a6f9f33f625bb4da6d168927a939386147e069219343 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | http://dx.doi.org/10.4322/rbpv.01804004 |
| PMID | 20040204 |
| PQID | 733638358 |
| PQPubID | 23479 |
| PageCount | 6 |
| ParticipantIDs | scielo_journals_S1984_29612009000400004 proquest_miscellaneous_733638358 crossref_primary_10_4322_rbpv_01804004 pubmed_primary_20040204 |
| PublicationCentury | 2000 |
| PublicationDate | 2009-10-01 |
| PublicationDateYYYYMMDD | 2009-10-01 |
| PublicationDate_xml | – month: 10 year: 2009 text: 2009-10-01 day: 01 |
| PublicationDecade | 2000 |
| PublicationPlace | Brazil |
| PublicationPlace_xml | – name: Brazil |
| PublicationTitle | Revista brasileira de parasitologia veterinaria |
| PublicationTitleAlternate | Rev Bras Parasitol Vet |
| PublicationYear | 2009 |
| Publisher | Colégio Brasileiro de Parasitologia Veterinária |
| Publisher_xml | – name: Colégio Brasileiro de Parasitologia Veterinária |
| References | INOKUMA, H. 2001; 63 KOCAN, A. 2000; 6 THOMPSON, J. D. 1997; 25 BULLA, C. 2004; 35 ALLSOPP, M. T. E.; ALSOPP, B. A. 2001; 39 SAITOU, N.; NEI, M. 1897; 4 DU PLESSIS, J. L. 1990; 57 DANTAS-TORRES, F. 2008; 1 CASTRO, M. B. 2004; 119 MASSUNG, R. 1998; 36 KORDICK, S. K. 1999; 37 SUKASAWAT, J. 2001; 39 CALIC, S. B. 2004; 8 OLIVEIRA, L. 2009 WANER, T. 2001; 95 COSTA, P. S. G.; BRIGATTE, M. E.; GRECO, D. B. 2005; 100 UNVER, A. 2001; 39 COSTA Jr., L. M. 2007; 174 MACIEIRA, D. B. 2005; 34 CARLOS, R. S. 2007; 16 WALKER, D. H.; DUMLER, J. S. 1996 DINIZ, P. P. 2008; 22 AGUIAR, D. M. 2007; 37 PREOZI, D. E.; COHN, L. A. 2002; 24 COSTA, P. S. G. 2006; 10 LABRUNA, M. B. 2007; 143 HEADLEY, S. A. 2006; 12 SOARES, A. O. 2006; 15 AGUIAR, D. M.; HAGIWARA, M. K.; LABRUNA, M. B. 2008; 39 PERSING, D. H. 1966 SANTOS, F. 2009; 179 CARDOZO, G. P. 2009; 164 SAITO, T. B. 2008; 79 DUMLER, J. S. 2001; 51 TRAPP, S. M. 2006; 140 LABARTHE, N. 2003; 4 OYAFUSO, M. K. 2002; 23 MURPHY, G. L. 1998; 79 BREITSCHWERDT, E. B.; HEGARTY, B. C.; HANCOCK, S. I. 1998; 36 CARVALHO, F. S. 2008; 7 FELSENSTEIN, J. 1985; 39 OLIVEIRA, T. M. 2008; 17 NAKAGHI, A. C. H. 2008; 38 KUMAR, S. 2001; 17 CHAE, J. S. 2003; 990 HEEB, H. L. 2003; 39 SAMBROOK, J.; RUSSEL, D. W. 2001 CASPERSEN, K. 2002; 70 DAGNONE, A. S. 2003; 117 OLIVEIRA, D. 2000; 9 DE PAIVA DINIZ, P. P 2007; 7 |
| References_xml | – volume: 63 start-page: 815 issue: 7 year: 2001 end-page: 817 article-title: Detection of ehrlichial infection by PCR in dogs from Yamaguchi and Okinawa Prefectures, Japan publication-title: The Journal of Veterinary Medical Science contributor: fullname: INOKUMA, H. – volume: 16 start-page: 117 issue: 3 year: 2007 end-page: 120 article-title: Frequency of antibodies anti-Ehrlichia canis, Borrelia burgdorferi and Dirofilaria immitis antigens in dogs from microrregion Ilhéus - Itabuna, State of Bahia, Brazil publication-title: Revista Brasileira de Parasitologia Veterinária contributor: fullname: CARLOS, R. S. – volume: 174 start-page: 673 issue: 3 year: 2007 end-page: 6 article-title: Sero -prevalence and risk indicators for canine ehrlichiosis in three rural areas of Brazil publication-title: Veterinary Journal contributor: fullname: COSTA Jr., L. M. – volume: 140 start-page: 223 issue: 3 year: 2006 end-page: 230 article-title: Seroepidemiology of canine babesiosis and ehrlichiosis in a hospital population publication-title: Veterinary Parasitology contributor: fullname: TRAPP, S. M. – volume: 100 start-page: 853 issue: 8 year: 2005 end-page: 859 article-title: Antibodies to Rickettsia rickettsii, Rickettsia typhi, Coxiella burnetti, Bartonella henselae, Bartonella quintana, and Ehrlichia chaffeensis among healthy population in Minas Gerais, Brazil publication-title: Memórias do Instituto Oswaldo Cruz contributor: fullname: COSTA, P. S. G.; BRIGATTE, M. E.; GRECO, D. B. – volume: 39 start-page: 4204 issue: 11 year: 2001 end-page: 4207 article-title: Novel Ehrlichia genotype detected in dogs in South Africa publication-title: Journal of Clinical Microbiology contributor: fullname: ALLSOPP, M. T. E.; ALSOPP, B. A. – volume: 143 start-page: 189 issue: 2 year: 2007 end-page: 95 article-title: preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil publication-title: Veterinary Parasitology contributor: fullname: LABRUNA, M. B. – volume: 17 start-page: 1244 issue: 12 year: 2001 end-page: 1245 article-title: Mega2: Molecular evolutionary genetics analysis software publication-title: Bioinformatics contributor: fullname: KUMAR, S. – volume: 23 start-page: 87 issue: 1 year: 2002 end-page: 90 article-title: Characterization of ticks infecting dogs in a hospital population in North Paraná, Brazil publication-title: Semina- Ciências Agrárias contributor: fullname: OYAFUSO, M. K. – volume: 4 start-page: 67 issue: 1 year: 2003 end-page: 75 article-title: Serologic prevalence of Dirofilaria immitis, Ehrlichia canis, and Borrelia burgdorferi infections in Brazil publication-title: Veterinary Therapeutics contributor: fullname: LABARTHE, N. – volume: 24 start-page: 277 issue: 4 year: 2002 end-page: 288 article-title: The increasingly complicated story of Ehrlichia publication-title: Compendium on Continuing Education for the Practicing Veterinarian contributor: fullname: PREOZI, D. E.; COHN, L. A. – volume: 79 start-page: 102 issue: 1 year: 2008 end-page: 8 article-title: Canine infection by rickettsiae and ehrlichieae in southern Brazil publication-title: American Journal of Tropical Medicine and Hygiene contributor: fullname: SAITO, T. B. – volume: 39 start-page: 379 issue: 4 year: 2003 end-page: 384 article-title: Large granular lymphocytosis, lymphocyte subset inversion, thrombocytopenia, dysproteinemia, and positive Ehrlichia serology in a dog publication-title: Journal of the American Animal Hospital Association contributor: fullname: HEEB, H. L. – volume: 37 start-page: 796 issue: 3 year: 2007 end-page: 802 article-title: Serological diagnosis of canine monocytic ehrlichiosis with Brazilian antigen of Ehrlichia canis publication-title: Ciência Rural contributor: fullname: AGUIAR, D. M. – volume: 39 start-page: 90 issue: 1 year: 2001 end-page: 93 article-title: Coinfection with three Ehrlichia species in dogs from Thailand and Venezuela with emphasis on consideration of 16S ribossomal DNA secondary structure publication-title: Journal of Clinical Microbiology contributor: fullname: SUKASAWAT, J. – start-page: 18 issue: 2 year: 1996 end-page: 25 article-title: Emergence of the ehrlichiosis as human health problems publication-title: Emerging Infectious Diseases contributor: fullname: WALKER, D. H.; DUMLER, J. S. – volume: 37 start-page: 2631 issue: 8 year: 1999 end-page: 2638 article-title: Coinfection with multiple tick-borne pathogens in a Walker Hound Kennel in North Carolina publication-title: Journal of Clinical Microbiology contributor: fullname: KORDICK, S. K. – volume: 95 start-page: 1 issue: 1 year: 2001 end-page: 15 article-title: Significance of serological testing for ehrlichial diseases in dogs with special emphasis on the diagnosis of canine monocytic ehrlichiosis caused by Ehrlichia canis publication-title: Veterinary Parasitology contributor: fullname: WANER, T. – volume: 36 start-page: 2645 issue: 9 year: 1998 end-page: 2651 article-title: Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewiingii, or Bartonella vinsonii publication-title: Journal of Clinical Microbiology contributor: fullname: BREITSCHWERDT, E. B.; HEGARTY, B. C.; HANCOCK, S. I. – volume: 9 start-page: 1 issue: 1 year: 2000 end-page: 6 article-title: Detecção de anticorpos anti-Ehrlichia canis em cães naturalmente infectados, através do "DOT-ELISA" publication-title: Revista Brasileira de Parasitologia Veterinária contributor: fullname: OLIVEIRA, D. – volume: 990 start-page: 248 issue: 1 year: 2003 end-page: 256 article-title: Prevalence and sequence analyses of Neorickettsia risticii publication-title: Annals of the New York Academy of Sciences contributor: fullname: CHAE, J. S. – volume: 51 start-page: 2145 year: 2001 end-page: 2165 article-title: Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: identification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ‘HGE agent' as subjective synonyms of Ehrlichia phagocytophila publication-title: International Journal of Systematic and Evolutionary Microbiology contributor: fullname: DUMLER, J. S. – volume: 79 start-page: 325 issue: 4 year: 1998 end-page: 339 article-title: A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma publication-title: Veterinary Parasitology contributor: fullname: MURPHY, G. L. – volume: 34 start-page: 44 issue: 1 year: 2005 end-page: 48 article-title: Prevalence of Ehrlichia canis infection in thrombocytopenic dogs from Rio de Janeiro, Brazil publication-title: Veterinary Clinical Pathology contributor: fullname: MACIEIRA, D. B. – volume: 38 start-page: 776 issue: 3 year: 2008 end-page: 770 article-title: Clinical, hematological, serological and molecular survey of canine ehrlichiosis publication-title: Ciência Rural contributor: fullname: NAKAGHI, A. C. H. – volume: 36 start-page: 1090 issue: 4 year: 1998 end-page: 1095 article-title: Nested PCR assay for detection of Granulocytic Ehrlichiae publication-title: Journal of Clinical Microbiology contributor: fullname: MASSUNG, R. – volume: 1 start-page: 25 issue: 1 year: 2008 article-title: Canine vector-borne diseases in Brazil publication-title: erinary Parasitology contributor: fullname: DANTAS-TORRES, F. – volume: 22 start-page: 1136 issue: 5 year: 2008 end-page: 1143 article-title: Serum cardiac troponin I concentration in dogs with ehrlichiosis publication-title: Journal of Veterinary Internal Medicine contributor: fullname: DINIZ, P. P. – volume: 8 start-page: 259 issue: 3 year: 2004 end-page: 262 article-title: Human Ehrlichiosis in Brazil: first suspect cases publication-title: The Brazilian Journal of Infectious Diseases contributor: fullname: CALIC, S. B. – year: 1966 publication-title: PCR Protocols for Emerging Infectious Diseases: A supplement to Diagnostic Molecular Microbiology: Principles and Applications contributor: fullname: PERSING, D. H. – year: 2001 publication-title: Molecular cloning: a laboratory manual contributor: fullname: SAMBROOK, J.; RUSSEL, D. W. – volume: 7 start-page: 657 issue: 3 year: 2008 end-page: 62 article-title: Epidemiological and molecular study of Ehrlichia canis in dogs in Bahia, Brazil publication-title: Genetics and Molecular Research contributor: fullname: CARVALHO, F. S. – volume: 70 start-page: 1230 issue: 3 year: 2002 end-page: 1234 article-title: Genetic variability and stability of Anaplasma phagocytophila msp2 (p44) publication-title: Infection and Immunity contributor: fullname: CASPERSEN, K. – volume: 12 start-page: 1303 issue: 8 year: 2006 end-page: 1304 article-title: Neorickettsia helminthoeca in dog, Brazil publication-title: Emerging Infectious Diseases contributor: fullname: HEADLEY, S. A. – volume: 39 start-page: 489 issue: 3 year: 2008 end-page: 493 article-title: In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil publication-title: Brazilian Journal of Microbiology contributor: fullname: AGUIAR, D. M.; HAGIWARA, M. K.; LABRUNA, M. B. – volume: 35 start-page: 141 issue: 1 year: 2004 end-page: 6 article-title: The relationship between the degree of thrombocytopenia and infection with Ehrlichia canis in an endemic area publication-title: Veterinary Research contributor: fullname: BULLA, C. – volume: 25 start-page: 4876 issue: 24 year: 1997 end-page: 4882 article-title: The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools publication-title: Nucleic Acids Research contributor: fullname: THOMPSON, J. D. – volume: 15 start-page: 13 issue: 1 year: 2006 end-page: 6 article-title: Evaluation of ectoparasites and hemoparasites in dogs kept in apartments and houses with yards in the city of Juiz de Fora, Minas Gerais, Brazil publication-title: Revista Brasileira de Parasitologia Veterinária contributor: fullname: SOARES, A. O. – year: 2009 article-title: First report of Ehrlichia ewingii detected by molecular investigation in dogs from Brazil publication-title: Clinical Microbiology and Infection contributor: fullname: OLIVEIRA, L. – volume: 179 start-page: 145 issue: 1 year: 2009 end-page: 148 article-title: Molecular evaluation of the incidence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs from Ribeirão Preto, Brazil publication-title: Veterinary Journal contributor: fullname: SANTOS, F. – volume: 119 start-page: 73 issue: 1 year: 2004 end-page: 86 article-title: Experimental acute canine monocytic ehrlichiosis: clinicopathological and immunopathological findings publication-title: Veterinary Parasitology contributor: fullname: CASTRO, M. B. – volume: 39 start-page: 2788 issue: 8 year: 2001 end-page: 2793 article-title: Molecular and antigenic comparasion of Ehrlichia canis isolates from dogs, ticks, and a human in Venezuela publication-title: Journal of Clinical Microbiology contributor: fullname: UNVER, A. – volume: 117 start-page: 285 issue: 4 year: 2003 end-page: 290 article-title: Ehrlichiosis in anemic, thrombocytopenic, or tick-infested dogs from a hospital population in South Brazil publication-title: Veterinary Parasitology contributor: fullname: DAGNONE, A. S. – volume: 39 start-page: 783 issue: 4 year: 1985 end-page: 791 article-title: Confidence limits on phylogenies: an approach using the bootstrap publication-title: Evolution contributor: fullname: FELSENSTEIN, J. – volume: 10 start-page: 7 issue: 1 year: 2006 end-page: 10 article-title: More about Human Monocytotropic Ehrlichiosis in Brazil: serological evidence of new nine cases publication-title: The Brazilian Journal of Infectious Diseases contributor: fullname: COSTA, P. S. G. – volume: 164 start-page: 338 issue: 11 year: 2009 end-page: 340 article-title: Molecular characterization of two strains of Anaplasma platys in Brazil publication-title: Veterinary Records contributor: fullname: CARDOZO, G. P. – volume: 6 start-page: 477 issue: 5 year: 2000 end-page: 480 article-title: Naturally occurring Ehrlichia chaffeensis infection in coyotes from Oklahoma publication-title: Emerging Infectious Diseases contributor: fullname: KOCAN, A. – volume: 57 start-page: 151 issue: 3 year: 1990 end-page: 155 article-title: Concurrent babesiosis and ehrlichiosis in the dog: blood smear examination supplemented by the indirect fluorescent antibody test, using Cowdria ruminantium as antigen publication-title: Onderspoort Journal of Veterinary Research contributor: fullname: DU PLESSIS, J. L. – volume: 7 start-page: 689 issue: 4 year: 2007 end-page: 697 article-title: Surveillance for zoonotic vector-borne infections using sick dogs from southeastern Brazil publication-title: Vector Borne and Zoonotic Diseases contributor: fullname: DE PAIVA DINIZ, P. P – volume: 17 start-page: 7 issue: 1 year: 2008 end-page: 11 article-title: A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and Ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test publication-title: Revista Brasileira de Parasitologia Veterinária contributor: fullname: OLIVEIRA, T. M. – volume: 4 start-page: 406 issue: 4 year: 1897 end-page: 425 article-title: The neighbor-joining method: a new method for reconstructing phylogenetic trees publication-title: Molecular Biology and Evolution contributor: fullname: SAITOU, N.; NEI, M. |
| SSID | ssj0060003 |
| Score | 1.9906622 |
| Snippet | Ehrlichioses are important emerging zoonotic tick-borne diseases that can affect both animals and humans. Clinical manifestations of ehrlichiosis caused by... |
| SourceID | scielo proquest crossref pubmed |
| SourceType | Open Access Repository Aggregation Database Index Database |
| StartPage | 20 |
| SubjectTerms | Anaplasmataceae - genetics Anaplasmataceae Infections - blood Anaplasmataceae Infections - diagnosis Anaplasmataceae Infections - veterinary Animals DNA, Bacterial - blood Dog Diseases - blood Dog Diseases - diagnosis Dogs Molecular Diagnostic Techniques PARASITOLOGY VETERINARY SCIENCES |
| Title | Molecular diagnosis of Anaplasmataceae organisms in dogs with clinical and microscopical signs of ehrlichiosis |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/20040204 https://search.proquest.com/docview/733638358 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612009000400004&lng=en&tlng=en |
| Volume | 18 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3Na9swFBdpd9llbN1Xug90GNvBeHMsx5aOZUnpoO1gSUfYxciWnBhcO8RNoP3r954lO0k32HbYRQRhXoTej_el90HIO5kM1DDlyg19X7sBSzw3Sb3UDbMsGUotmGxC2WeT6HLGR-Ng3Ou1k0C3e_-V07AHvMbK2X_gdkcUNuA38BxW4Dqsf8X3i3beLYZVMYvONBw5KeUSDGWwT2WqZTvNqb426bDV3Fa5dYWSGE-_xmQ9LFsxpZP53KTM6cWqyNNFjqR3bVt8ZgBb0wH_uwZZk6-ko7SDvcXrvJmSm0tng9k3WAKcd-pghKe0cdVSOpPm6X5UbLZfTKr1nQn_Fko6XzANrTBjzW0EQ9n3flt8lFa1862am72VSTSzUfeFVJVJKK-xqqKQzg-Z4mn2wx-iS6TrIqIec8GImv1epG_jFUY-ezua3lRc39chAYg4YPIqWW4-YnczFHJbZdkmCFx-jU-vzs_j6Xg2PSAPfBBznUNv7IAQ3c0mgdYe0HR4RfKf9ojvW0S_uDnYwxbEfLFr90wfk0fWYaEnBmlPSE-XR-Toe8vHW3phszOekrLDHu2wR6uM3sMe7bBH85Ii9ihij7bYo4A9uoc92mAPSe1i7xm5Oh1PP5-5dp6HmzLPu3FZMuQ4ehNbzEUskzzwdSTDTGSMZeCHJ0mgZKgGIRd-JAUTjIPxGGkvFKBXWcCek0ME5EtCORdc6izyUyGCFI3egGcKrE3N_GSooj55395pvDRtW2Jwd_HyY7z8uL38PqHtjccgWPG1TJa6Wtcx9gll4J_wPnlhONFRQnmDReV98sGwJrYCoI4nA8GD2BfgOABYvUZHwnL85795RR5u8f2aHN6s1voNOajV-m2Dq58rhrAD |
| link.rule.ids | 230,315,782,786,866,887,27933,27934 |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Molecular+diagnosis+of+Anaplasmataceae+organisms+in+dogs+with+clinical+and+microscopical+signs+of+ehrlichiosis&rft.jtitle=Revista+brasileira+de+parasitologia+veterinaria&rft.au=Dagnone%2C+Ana+S%C3%ADlvia&rft.au=Souza%2C+Alda+Izabel+de&rft.au=Andr%C3%A9%2C+Marcos+Rog%C3%A9rio&rft.au=Machado%2C+Rosangela+Zacarias&rft.date=2009-10-01&rft.issn=0103-846X&rft.volume=18&rft.issue=4&rft.spage=20&rft.epage=25&rft_id=info:doi/10.4322%2Frbpv.01804004&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0103-846X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0103-846X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0103-846X&client=summon |