Analysis of dendritic cells at the genetic level

• Published in: Advances in experimental medicine and biology Vol. 417; p. 443
• Main Authors: Marland, G, Hartgers, F C, Veltkamp, R, Königswieser, M F, Gorman, D, McClanahan, T, Figdor, C G, Adema, G J
• Format: Journal Article
• Language: English
• Published: United States 1997
• Subjects: Antigen Presentation - genetics; Dendritic Cells - immunology; Dendritic Cells - metabolism; DNA, Complementary - genetics; Gene Expression; Gene Library; Humans; In Vitro Techniques; Lectins, C-Type; Mannose-Binding Lectins; Polymerase Chain Reaction - methods; Receptors, Cell Surface - genetics
• ISSN: 0065-2598
• DOI: 10.1007/978-1-4757-9966-8_73

To increase our understanding of dendritic cell (DC) function we have used two approaches to search at the genetic level for molecules which are specifically expressed by these cells. First, we have performed random sequencing of cDNA libraries prepared from DC. Second, we have employed differential display PCR (DD-PCR). DD-PCR is a powerful technique for the identification at the RNA level of molecules which are expressed in a cell type-specific manner. In our study, we have compared RNA from DC with RNA from a panel of leukocyte cell lines. Here we present a summary of our findings using these two approaches, and show that both methods are complementary and can be used to identify molecules that are specific to DC.