Cloning and sequence of cDNA for human placental cytokeratin 8. Regulation of the mRNA in trophoblastic cells by cAMP

• Published in: Molecular endocrinology (Baltimore, Md.) Vol. 4; no. 3; p. 370
• Main Authors: Yamamoto, R, Kao, L C, McKnight, C E, Strauss, 3rd, J F
• Format: Journal Article
• Language: English
• Published: United States 01-03-1990
• Subjects: Amino Acid Sequence; Base Sequence; Cells, Cultured; Choriocarcinoma - genetics; Choriocarcinoma - metabolism; Choriocarcinoma - pathology; Cloning, Molecular; Cyclic AMP - pharmacology; DNA - genetics; Female; Gene Expression Regulation - drug effects; Humans; Keratins - genetics; Keratins - metabolism; Molecular Sequence Data; Placenta - metabolism; Pregnancy - metabolism; RNA, Messenger - drug effects; RNA, Messenger - genetics; RNA, Messenger - metabolism; Trophoblasts - cytology; Trophoblasts - drug effects; Trophoblasts - metabolism; Tumor Cells, Cultured - drug effects; Tumor Cells, Cultured - metabolism; Tumor Cells, Cultured - pathology
• ISSN: 0888-8809
• DOI: 10.1210/mend-4-3-370

A 1735 bp cDNA for human placental cytokeratin 8 is described which encompasses the entire coding sequence as well as 33 and 250 base pairs of 5'- and 3'-untranslated region, respectively. The level of cytokeratin 8 mRNA in various fetal tissues and placentae of different gestational ages was determined as were the effects of 8-bromo-cAMP on cytokeratin 8 mRNA in primary cultures of cytotrophoblasts and JEG-3 choriocarcinoma cells. Cytokeratin 8 mRNA was abundant in fetal small intestine, placenta, pancreas, lung, liver, and kidney. Levels of cytokeratin 8 mRNA in placenta increased slightly during pregnancy. 8-Bromo-cAMP suppressed cytokeratin 8 mRNA in primary cultures of cytotrophoblasts, whereas the cAMP analog increased mRNA levels in JEG-3 cells, revealing differential regulation of this mRNA in normal and transformed trophoblastic cells.