Extraction of Trigonella foenum-graecum L. by supercritical fluid CO₂ and its contact toxicity to Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae)

• Published in: Journal of pest science Vol. 80; no. 3; pp. 151 - 157
• Main Authors: Tang, G. W, Yang, C. J, Xie, L. D
• Format: Journal Article
• Language: English
• Published: Heidelberg Berlin/Heidelberg : Springer-Verlag 01-08-2007; Springer Nature B.V
• Subjects: Alcohols; Biocompatibility; Carbon dioxide; Contact toxicity; Filter paper; In vivo methods and tests; Orthogonal experiment; Pesticide resistance; Pesticides; Rhyzopertha dominica; Rhyzopertha dominica (Fabricius); Seeds; Supercritical extraction of CO; Supercritical fluids; Time dependence; Toxicity; Trigonella foenum-graecum; Trigonella foenum-graecum L
• ISSN: 1612-4758; 1612-4766
• DOI: 10.1007/s10340-007-0167-8

Rhyzopertha dominica (Fabricius) has developed extensive pesticide resistance in the last several decades. We have developed a supercritical fluid extraction method for Trigonella foenum-graecum L. (TFG) and studied the contact toxicities of the extracts to R. dominica. The extraction method was designed with orthogonal experiments to preserve and collect all the possible active components. Contact toxicity and efficiency of extraction were used as standard values to optimize extraction conditions, which were achieved at 55°C under 25 Mpa of pressure. The extraction efficiency for 200 g of dry sample reached 6.21% with 30 ml of 95% alcohol. Extracts loaded on filter paper showed dose and time dependent toxicities to adult R. dominica with a LC₅₀ value of 65.02 μg/cm² after 3 days post treatment. Our extensive in vivo studies indicated the extracts from Trigonella foenum-graecum seeds have high efficacy against pesticide resistant R. dominica. The active ingredient(s) from the extract shows promise as a novel pesticide candidate.