Effect of NtDCN1 gene activated during embyogenesis on organogenesis in tobacco tissue culture

• Published in: Russian journal of plant physiology Vol. 57; no. 1; pp. 118 - 123
• Main Authors: Dolgikh, Yu. I, Stepanova, A. Yu, Osipova, E. S, Tashpulatov, A. Sh, Hosp, J, Ribarits, A, Heberle-Bors, E, Touraev, A. M
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 2010; SP MAIK Nauka/Interperiodica
• Subjects: auxin; Auxins; Biomedical and Life Sciences; Hormones; Leaves; Life Sciences; Nicotiana tabacum; NtDCN1 gene; Organogenesis; Plant Physiology; Plant Sciences; Research Papers; Roots; Shoots; Somatic embryogenesis; Tissue culture; auxin; Nicotiana tabacum; organogenesis; NtDCN1 gene
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443710010164

The role of NtDCN1 gene in organogenesis in the culture of tobacco (Nicotiana tabacum L.) somatic tissues was studied. This gene is specifically expressed in tobacco microspores induced for somatic embryogenesis. This gene knockout resulted in a disturbance of formation and development of embyoids from microspores. In leaf disks and calli derived from tobacco lines with active and inactivated NtDCN1 gene, we studied induction of shoots and roots. A comparative analysis of tobacco line morphogenetic responses in vitro showed that NtDCN1 gene inactivation enhanced shoot formation and suppressed rhizogenesis, whereas this gene reactivation returned organogenesis processes to control level. Difference between lines was manifested only at a definite ratio between exogenous hormones supplied. The involvement of NtDCN1 gene in line responses to exogenous auxin is discussed. The results obtained permit a supposition that the NtDCN1 gene is critical for regulation not only somatic embryogenesis but also organogenesis.