Mechanism of the Escherichia coli DNA T:G-mismatch Endonuclease (Vsr protein) Probed with Thiophosphate-containing Oligodeoxynucleotides

The mechanism of the Escherichia coli DNA T:G mismatch endonuclease (Vsr) has been investigated using oligodeoxynucleotides substituted, at the scissile phosphate, with isomeric phosphorothioates and a 3′-phosphorothiolate. Binding and kinetic data with the phosphorothioates/phosphorothiolate indica...

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Bibliographic Details
Published in:	Journal of molecular biology Vol. 353; no. 3; pp. 692 - 703
Main Authors:	Elliott, Sarah L., Brazier, John, Cosstick, Richard, Connolly, Bernard A.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 28-10-2005
Subjects:	Base Pairing Base Sequence DNA, Bacterial - chemistry DNA, Bacterial - genetics Endodeoxyribonucleases - chemistry Escherichia coli Escherichia coli - genetics Hydrolysis Kinetics Molecular Probes nuclease Oligodeoxyribonucleotides - chemistry Phosphates - chemistry phosphodiester bond hydrolysis phosphorothioates phosphorothiolates Stereoisomerism Vsr endonuclease MALDI-TOF MS phosphorothioates nuclease Vsr endonuclease phosphodiester bond hydrolysis phosphorothiolates PLD
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