Oral Administration of Interleukin-10 and Anti-IL-1 Antibody Ameliorates Experimental Intestinal Inflammation

• Published in: Gastroenterology research Vol. 6; no. 4; pp. 124 - 133
• Main Authors: Cardani, Diego, Dusio, Giuseppina F, Luchini, Patrizia, Sciarabba, Michele, Solimene, Umberto, Rumio, Cristiano
• Format: Journal Article
• Language: English
• Published: Canada Elmer Press 01-08-2013
• Subjects: Original; Low doses; Interleukin-10; Tight-junctions; Anti-interleukin-1 alpha antibody; Intestinal bowel disease
• ISSN: 1918-2805; 1918-2813
• DOI: 10.4021/gr556w

To elucidate the effects of a solution containing interleukin-10 and anti-IL-1 antibody in modulating experimental intestinal inflammation.
Colitis was induced in BALB/c mice by oral administration of dextran sodium sulphate; mice were then treated with interleukin-10 plus anti-IL-1 antibody at low dosage. Transepithelial electrical resistance of isolated mouse colon and colon lengths were evaluated. Cytokines concentrations in organocultures supernatants and plasma samples were evaluated by Enzyme-Linked Immuno Sorbent Assay. Tight junction proteins were evaluated by immunofluorescence, respectively.
Oral administration of tested products restores intestinal barrier function during experimental intestinal inflammation in association with reduced levels of proinflammatory cytokines, increased interleukin-10 plasma concentrations and a tight junction architecture restoration.
Obtained results may contribute to modelling an interesting strategy for the treatment of patients with inflammatory bowel diseases.