Multiplexed RT- PCR for high throughput screening applications

To increase efficiency of high throughput gene expression profiling, we established a new TaqMan RT-PCR (real-time reverse transcriptase-polymerase chain reaction with internal probes for the quantification of PCR products) method for quantitative mRNA expression analysis. In this procedure, we util...

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Bibliographic Details

Published in:	Combinatorial chemistry & high throughput screening Vol. 7; no. 8; p. 727
Main Authors:	Maley, Derrick, Mei, Jay, Lu, Hong, Johnson, Dana L, Ilyin, Sergey E
Format:	Journal Article
Language:	English
Published:	United Arab Emirates 01-12-2004
Subjects:	Cell Count Cells, Cultured Gene Expression Profiling - methods Glyceraldehyde-3-Phosphate Dehydrogenases - genetics Humans K562 Cells Poly A - genetics Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - biosynthesis RNA, Messenger - genetics RNA, Messenger - isolation & purification
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