Eradication of Klebsiella pneumoniae biofilms and persister cells using silver nitrate

• Published in: Kuwait journal of science Vol. 50; no. 1 A; pp. 1 - 8
• Main Authors: Aziz, Sarah Naji, al-Marjani, Muhammad F.
• Format: Journal Article
• Language: English
• Published: Kuwait Kuwait University, Academic Publication Council 01-01-2023
• ISSN: 2307-4108; 2307-4116
• DOI: 10.48129/kjs.15153

To perceive the relationship between the emergences of antibiotics persistence, biofilm formation, and to study the role of silver nitrate in fighting these microbes, a total 50 clinical Klebsiella pneumonae isolates were collected from clinical sources and identified using genotypic identification by using specific primer (rpoB gene) from housekeeping genes. Detection of their biofilm-forming abilities were carried out. The eradication effect of silver nitrate to control bacterial persistence and reducing the biofilm-forming abilities were studied. We found that 40 isolate (80%) were biofilm-formers. From bacterial persisters, (75%) were biofilm-formers. Silver nitrate showed a great role to eradicate persister cells and inhibition of bacterial biofilm formation of clinical isolates of K. pneumoniae.