Responsiveness of Candidate Genes on CoPv01CDRK/PhgPv01CDRK Loci in Common Bean Challenged by Anthracnose and Angular Leaf Spot Pathogens

• Published in: International journal of molecular sciences Vol. 24; no. 22; p. 16023
• Main Authors: Lovatto, Maike, Gonçalves-Vidigal, Maria Celeste, Vaz Bisneta, Mariana, Calvi, Alexandre Catto, Mazucheli, Josmar, Vidigal Filho, Pedro Soares, Miranda, Eduardo Gomes Rosa, Melotto, Maeli
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 07-11-2023
• Subjects: Beans; candidate gene expression; Chromosomes; common bean–angular leaf spot interaction; common bean–anthracnose interaction; CoPv01CDRK/PhgPv01CDRK loci; Cultivars; Gene expression; Kinases; Pathogens; plant defense genes; Proteins; United States > US; California
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms242216023

Anthracnose (ANT) and angular leaf spot (ALS) are significant diseases in common bean, leading to considerable yield losses under specific environmental conditions. The California Dark Red Kidney (CDRK) bean cultivar is known for its resistance to multiple races of both pathogens. Previous studies have identified the CoPv01CDRK/PhgPv01CDRK resistance loci on chromosome Pv01. Here, we evaluated the expression levels of ten candidate genes near the CoPv01CDRK/PhgPv01CDRK loci and plant defense genes using quantitative real-time PCR in CDRK cultivar inoculated with races 73 of Colletotrichum lindemuthianum and 63-39 of Pseudocercospora griseola. Gene expression analysis revealed that the Phvul.001G246300 gene exhibited the most elevated levels, showing remarkable 7.8-fold and 8.5-fold increases for ANT and ALS, respectively. The Phvul.001G246300 gene encodes an abscisic acid (ABA) receptor with pyrabactin resistance, PYR1-like (PYL) protein, which plays a central role in the crosstalk between ABA and jasmonic acid responses. Interestingly, our results also showed that the other defense genes were initially activated. These findings provide critical insights into the molecular mechanisms underlying plant defense against these diseases and could contribute to the development of more effective disease management strategies in the future.