Effect of monoclonal antihuman IgE on recombinant IgE(301-376) inhibition of specific IgE histamine release

To explore the binding domains of rCIgE(301-376) necessary for inhibition of passive transfer of rye grass and Chinese elm IgE to human basophils, we employed monoclonal antibodies known to bind to IgE(301-336), M-272, and to IgE(367-376), M-27. By preincubating M-272, but not M-27, with rCIgE(301-3...

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Bibliographic Details
Published in:	International archives of allergy and applied immunology Vol. 88; no. 1-2; p. 203
Main Authors:	Glovsky, M M, Kebo, D, Helm, B A, Horvath, S, Richards, J A, Chretien, I, Bencherau, J, Gould, H
Format:	Journal Article
Language:	English
Published:	Switzerland 01-01-1989
Subjects:	Allergens Antibodies, Anti-Idiotypic - immunology Antibodies, Monoclonal - immunology Antigen-Antibody Reactions Antigens, Differentiation, B-Lymphocyte - metabolism Basophils - immunology Histamine Release Humans Immunization, Passive Immunoglobulin E - immunology In Vitro Techniques Peptide Fragments Receptors, Fc - metabolism Receptors, IgE Recombinant Proteins - immunology Structure-Activity Relationship Trees
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Summary:	To explore the binding domains of rCIgE(301-376) necessary for inhibition of passive transfer of rye grass and Chinese elm IgE to human basophils, we employed monoclonal antibodies known to bind to IgE(301-336), M-272, and to IgE(367-376), M-27. By preincubating M-272, but not M-27, with rCIgE(301-376), passive transfer of specific IgE to basophils was partially inhibited. This implies that M-272 recognizes a binding site on rCIgE(301-376) or sterically interferes with the rCIgE(301-376) high-affinity binding domains on human basophils.
ISSN:	0020-5915
DOI:	10.1159/000234786