Enumerating antigen-specific T-cell responses in peripheral blood: A comparison of peptide MHC tetramer, ELISpot, and intracellular cytokine analysis

Detection of the circulating antigen-specific T-cell response to immunization is an important biologic end point in clinical trials of cancer vaccines. Typically employed assays are peptide MHC tetramer, ELISpot, and intracellular cytokine analysis. Although there is no agreement on the definition o...

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Bibliographic Details
Published in:	Journal of immunotherapy (1997) Vol. 28; no. 1; pp. 63 - 72
Main Authors:	HOBEIKA, Amy C, MORSE, Michael A, OSADA, Takuya, GHANAYEM, Manar, NIEDZWIECKI, Donna, BARRIER, Robert, LYERLY, H. Kim, CLAY, Timothy M
Format:	Journal Article
Language:	English
Published:	Hagerstown, MD Lippincott 2005 Philadelphia,PA
Subjects:	Antigens - immunology Antigens, CD - analysis Antigens, Differentiation, T-Lymphocyte - analysis Antineoplastic agents Biological and medical sciences CD8-Positive T-Lymphocytes - immunology Cytomegalovirus - immunology Cytomegalovirus Infections - blood Cytomegalovirus Infections - immunology Enzyme-Linked Immunosorbent Assay - methods Flow Cytometry Genes, MHC Class I - immunology HLA-A Antigens - immunology HLA-A2 Antigen Humans Immunologic Tests - methods Immunologic Tests - statistics & numerical data Immunotherapy Interferon-gamma - metabolism Lectins, C-Type Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - metabolism Lymphocyte Activation - immunology Medical sciences Peptide Fragments - immunology Pharmacology. Drug treatments Phosphoproteins - blood Phosphoproteins - immunology Reproducibility of Results Serologic Tests - methods T-Lymphocytes - cytology T-Lymphocytes - immunology T-Lymphocytes - metabolism Viral Matrix Proteins - blood Viral Matrix Proteins - immunology Cytomegalovirus Biological fluid Immune response Peptides Herpesviridae Major histocompatibility system T cell Cytokine peptide MHC tetramer Enzyme immunoassay Betaherpesvirinae Immunity CMV Blood ELISpot protective immunity Virus Antigen Prevention Immunotherapy Tetramer T-Lymphocyte Intracellular Comparative study
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