A comparison of genetic relationship measures in strawberry (Fragaria x ananassa Duch.) based on AFLPs, RAPDs, and pedigree data

• Published in: Euphytica Vol. 117; no. 1; pp. 1 - 12
• Main Authors: DEGANI, Chemda, ROWLAND, Lisa J, SAUNDERS, James A, HOKANSON, Stan C, OGDEN, Elizabeth L, GOLAN-GOLDHIRSH, Avi, GALLETTA, Gene J
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 2001; Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Cultivars; Cultivars: description, identification, tests, catalogs; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetic resources, diversity; Genetics; Genetics and breeding of economic plants; Plant material; Varietal selection. Specialized plant breeding, plant breeding aims; Canada; United States; North America; America; Amplified fragment length polymorphism; Genetic marker; Small fruits; Varietal identification; Phylogenetic tree; Rosaceae; Fragaria ananassa; Genetic distance; Random amplified polymorphic DNA marker; Dicotyledones; Angiospermae; Spermatophyta; Intraspecific comparison; Cultivar
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1023/A:1004008408435

Nineteen of the major strawberry (Fragaria × ananassa Duch.) cultivars grown in the UnitedStates and Canada were examined for AFLP markerpolymorphisms. For the AFLP reactions, the EcoRI-ACC primer was used in combination with fourMseI primers (MseI-CAC, MseI-CAG,MseI-CAT, or MseI-CTT). Each set ofprimers produced 46-66 scorable fragments ranging insize between 50 and 500 bp. The polymorphic fragmentsproduced from each set of primers were more thansufficient to distinguish among all the cultivars,demonstrating the usefulness of AFLP markers forcultivar identification. Similarity coefficients werecalculated based on data from 228 AFLP markers anddata from 15 previously characterized RAPD markers. The RAPD markers had been specifically selected forfingerprinting purposes because they succesfullydistinguish 41 strawberry cultivars, including the 19cultivars analyzed in this study. Separatedendrograms were constructed based on analysis of theAFLP and RAPD marker data using a neighbor-joiningalgorithm. The dendrograms were compared and found tobe very different. Correlations between similaritycoefficients calculated from AFLP marker data,similarity coefficients calculated from RAPD markerdata, and coefficients of coancestry calculated frompedigree information were evaluated. Interestingly,a better correlation with the coefficients ofcoancestry was observed with the RAPD marker data thanwith the AFLP marker data.[PUBLICATION ABSTRACT]