Effect of short-chain fatty acids on contractile activity and fluid flow in rat colon in vitro

• Published in: The American journal of physiology Vol. 262; no. 5 Pt 1; pp. G813 - G817
• Main Authors: Squires, P E, Rumsey, R D, Edwards, C A, Read, N W
• Format: Journal Article
• Language: English
• Published: United States 01-05-1992
• Subjects: Animals; Body Fluids - metabolism; Colon - drug effects; Colon - metabolism; Fatty Acids - chemistry; Fatty Acids - pharmacology; Gastrointestinal Motility - drug effects; In Vitro Techniques; Isotonic Solutions - pharmacology; Male; Osmolar Concentration; Rats; Rats, Inbred Strains
• ISSN: 0002-9513
• DOI: 10.1152/ajpgi.1992.262.5.g813

The effect of short-chain fatty acids (SCFAs) on the contractile activity and fluid output of the large bowel of the rat was studied using an isolated segment of cecum and colon, mounted in vitro. The rate of contractile activity per minute in the proximal, mid, and distal regions of the colon was depressed by luminal infusion of associated SCFAs either as a mixture (acetic, propionic, and butyric) or individually (100 mM/pH = 4.1, in each case). Dose responses were observed for the individual fatty acids, with the 100 mM solutions eliciting a more prominent reduction in colonic motor activity than that induced by 10 mM. Neither the Na salt of the fatty acids nor an acidified Krebs solution (pH = 4.1) inhibited contractile activity or fluid output. No reduction in the rate of contractile activity was observed in the cecum with any test solutions, except 100 mM butyric acid. The data suggest that SCFAs inhibit smooth muscle contractility and resultant fluid transit.