Quantitative trait loci affecting amylose, amylopectin and starch content in maize recombinant inbred lines

• Published in: Plant physiology and biochemistry Vol. 38; no. 6; pp. 459 - 472
• Main Authors: Séne, Mouhamadou, Causse, Mathilde, Damerval, Catherine, Thévenot, Claudine, Prioul, Jean-Louis
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 2000; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Biochemistry, Molecular Biology; Biological and medical sciences; Candidate gene; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; invertases; kernel; Life Sciences; maize; Metabolism; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; QTL; Shrunken 2; Varietal selection. Specialized plant breeding, plant breeding aims; Yield, quality, earliness, varia; RIL, recombinant inbred line; AGPase, ADPglucose-pyrophosphorylase; kernel; QTL; Starch.I, insoluble starch; AM, amylose; AP, amylopectin; Starch.T, total starch; QTL, quantitative trait locus; maize; cM, centiMorgan; WSF, water soluble fraction; IVR1, IVR2, soluble invertase genes; SPS, sucrose phosphate synthase; Shrunken 2; DW, dry weight; invertases; Candidate gene; WX, waxy; INCW1, INCW2, cell wall invertase genes; SH2, Shrunken 2; Genetic mapping; Monocotyledones; Zea mays; Genetic marker; Starch; Quantitative character; Chromosome; Cereal crop; Restriction fragment length polymorphism; Gramineae; Angiospermae; Spermatophyta; Chemical composition; Transgenic plant; Caryopsis; Biological accumulation
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/S0981-9428(00)00762-2

The loci explaining the variability of quantitative traits related to starch content and composition (amylose, amylopectin and water soluble fraction) were searched for in maize kernels. Multifactorial genetic methods were used to detect and locate QTLs (quantitative trait loci) on a genetic map consisting mainly of RFLP markers for genes with known function. The genetic material was recombinant inbred lines originating from parents differing in starch structure (dent vs. flint). Kernels were harvested from field grown plants for two successive years and under two pollination systems. Main effect and epistasis QTLs were detected using two methods, composite interval mapping (MQTL) and ANOVA. Despite large year-to-year differences, physiologically meaningful co-locations were observed between trait QTLs. Moreover, the number of expressed sequences on our map allowed the search for co-locations between QTLs and genes involved in carbohydrate metabolism. The main co-location was between an amylose QTL and
Shrunken 2 (SH2) locus, on chromosome 3 (SH2 encoding for the large subunit of ADPglucose pyrophosphorylase). The importance of this locus as a candidate gene for a starch QTL is in agreement with previous studies based either on QTL co-locations or on revertant analysis. Other co-locations were observed between amylose and amylopectin QTLs and the two loci of IVR1 invertase genes on chromosomes 2 and 10. Further comparison with previously detected QTLs for carbohydrate metabolism in maize leaves showed consistent co-location in map regions devoid of candidate genes, such as near chromosome 1S telomere. The possible contribution of regulatory genes in this region is discussed.