Fungal zoospore-mediated delivery of a foreign gene to wheat roots

• Published in: Phytopathology Vol. 84; no. 7; pp. 684 - 687
• Main Author: Zhang, Lingyu
• Format: Journal Article
• Language: English
• Published: St. Paul, MN American Phytopathological Society 1994
• Subjects: aciltransferasa; acyltransferase; acyltransferases; Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; esporas; expresion genica; expression des genes; Fundamental and applied biological sciences. Psychology; gene expression; gene transfer; genetic engineering; Genetic engineering applications; genetic transformation; Genetics and breeding of economic plants; genie genetique; ingenieria genetica; necrovirus de necrosis del tabaco; necrovirus necrose du tabac; olpidium brassicae; Plant breeding: fundamental aspects and methodology; racine; raices; roots; spore; spores; tobacco necrosis necrovirus; transferencia de genes; transfert de gene; transformacion genetica; transformation genetique; triticum aestivum; Recombinant microorganism; Biotechnology; Monocotyledones; Plant pathogen; Phycomycetes; Nucleoprotein; Cereal crop; Spores; Fungi; Proteins; Triticum; Transfection; Gene; Gramineae; Angiospermae; Transgenic plant; Chloramphenicol O-acetyltransferase; Vector; Thallophyta; Acyltransferases; Necrovirus; Genetic transformation; Root; Enzyme; Transferases; Tobacco necrosis virus; Plasmid; Virus; Encapsidation; Transmission electron microscopy; Olpidium brassicae; Genetic engineering; Spermatophyta; Capsid
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/Phyto-84-684

The primitive fungus Olpidium brassicae is an obligate plant parasite that acts as a natural virus vector transmitting tobacco necrosis virus and certain other viruses to roots of many monocotyledonous and dicotyledonous plants. Plasmid pAM981, carrying the chloramphenicol acetyltransferase (CAT) gene, was packaged by dissociated capsid protein of tobacco necrosis virus. The resulting nucleoprotein complexes were acquired by 0. brassicae zoospores and transmitted to wheat roots. Transient expression of CAT in wheat roots was detected, indicating that transformation can be achieved if plants can be regenerated from root tissue.