Olive oil increases the LIPC expression when associated with an Eastern pattern diet: An experimental study with Wistar rats

• Published in: Gene Vol. 887; p. 147738
• Main Authors: Bitencourt Brito, Priscila, Dalcin Teixeira, Mayza, Lehtonen Rodrigues de Souza, Ricardo, Furtado-Alle, Lupe, Viater Tureck, Luciane
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15-12-2023
• Subjects: APOB gene; APOE gene; Bioactive lipids; Gene expression modulation; LIPC gene; LIPC gene; HNF4α; apoE; PGC-1α; APOE gene; WC; APOB gene; ES; IDL; EVOO; HDL; MUFAs; LDL; VLDL; EPA; Bioactive lipids; DHA; WS; EC; Gene expression modulation
• DOI: 10.1016/j.gene.2023.147738
• EISSN: 1879-0038
• ISSN: 0378-1119

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•Consumption of extra virgin olive oil (EVOO) decreased food intake.•The interaction between the Eastern diet and EVOO led to LIPC expression increase.•EVOO and the Eastern diet had independent effects lowering triglyceride levels.•EVOO nutrigenomic effects were restricted to an Eastern human-based diet.
Some nutrigenomic effects of extra virgin olive oil (EVOO) are described in the literature; however, it is unknown whether its interaction with lipid-related genes is independent of the combined diet. In this sense, our objective was to investigate whether EVOO consumption associated with Western or Eastern human-based chow modulates the expression of APOE, APOB, and LIPC genes in rats. In view of this, the hypothesis is that the consumption of olive oil may not have the same nutrigenomic effects, depending on the diet consumed. For this study, 56 female rats were randomly divided into four groups: Western diet with EVOO (WS), Western-diet control (WC), Eastern-diet with EVOO (ES), and Eastern-diet control (EC). After 15 weeks, the animals were anesthetized with an intraperitoneal injection of chloral hydrate 15% (1.5 mL/kg) and euthanized by guillotining, and adipose tissue, liver, and blood were extracted. Triglycerides, cholesterol, and glucose levels were obtained following standard protocols, and relative gene expressions were calculated using the ΔΔCt method after quantitative PCR. The EVOO consumption was associated with LIPC gene expression increase in the liver only in animals fed the Eastern diet, compared to EC and WS animals. The EVOO consumption, combined with the Eastern diet, was associated with decreased triglyceride levels compared to WC. Although final weight and weight gain were similar between groups, WS animals had lower daily energy consumption. Conclusion: Given these results, the authors suggested that the EVOO nutrigenomic effects were restricted to an Eastern human-based diet.