Both Fc receptors and lymphocyte-function-associated antigen 1 on human T gamma lymphocytes are required for antibody-dependent cellular cytotoxicity (killer cell activity)

• Published in: European journal of immunology Vol. 14; no. 6; p. 518
• Main Authors: Miedema, F, Tetteroo, P A, Hesselink, W G, Werner, G, Spits, H, Melief, C J
• Format: Journal Article
• Language: English
• Published: Germany 1984
• Subjects: Antibodies, Monoclonal - immunology; Antibody-Dependent Cell Cytotoxicity; Antigens; Antigens, Surface - immunology; Cell Separation; Clone Cells; Enzyme-Linked Immunosorbent Assay; Humans; Killer Cells, Natural - immunology; Lymphocytosis - blood; Precipitin Tests; Receptors, Fc - immunology; Rosette Formation; T-Lymphocytes - classification; T-Lymphocytes - immunology
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830140607

A monoclonal antibody, designated CLB-LFA-1/1, directed to the human lymphocyte-function-associated antigen 1 (LFA-1) was raised by immunization of mice with the peripheral blood lymphocytes of a T gamma lymphocytosis patient. The monoclonal antibody was selected by inhibition of the natural killer cell and the antibody-dependent killer cell activity of the patient's T gamma lymphocytes. In addition, the monoclonal antibody was shown to inhibit the cytotoxic activity of T cell clones specific for either class I or class II HLA molecules. The antigen recognized by CLB-LFA-1/1 consisted of three polypeptide chains with molecular weights of 180 000 (alpha), 155 000 and 94 000 (beta). The antibody reacted with T cells, B cells, monocytes and granulocytes, and stained normal T gamma cells and T gamma cells of patients with T gamma lymphocytosis two- to threefold stronger than normal T cells. It was shown that LFA-1 and the Fc receptor on T gamma cells did not comodulate and it is therefore concluded that Fc receptors and LFA-1 are independent membrane structures, both required for the killer cell activity of T gamma cells.