Functional consequences of acute collagen degradation studied in crystalloid perfused rat hearts

• Published in: Basic research in cardiology Vol. 92; no. 3; pp. 147 - 158
• Main Authors: Todaka, K, Jiang, T, Chapman, J T, Gu, A, Zhu, S M, Herzog, E, Hochman, J S, Steinberg, S F, Burkhoff, D
• Format: Journal Article
• Language: English
• Published: Germany 01-06-1997
• Subjects: Animals; Body Water - metabolism; Collagen - metabolism; Crystalloid Solutions; Dithionitrobenzoic Acid - pharmacology; Extracellular Matrix - drug effects; Female; Heart - drug effects; In Vitro Techniques; Isotonic Solutions; Male; Myocardium - cytology; Myocardium - metabolism; Perfusion; Plasma Substitutes; Pressure; Rats; Rats, Sprague-Dawley; Time Factors; Vascular Resistance - drug effects; Ventricular Function, Left - drug effects
• ISSN: 0300-8428; 1435-1803
• DOI: 10.1007/BF00788632

The impact of acute collagen disruption by the disulfide donor, 5,5'-dithio-2-nitrobenzoic acid (DTNB) on ventricular properties was tested in rat hearts.
Collagen was degraded acutely in 13 isolated, isovolumically contracting rat hearts by perfusion with 1 mM DTNB added to Krebs-Henseleit solution for 1 hour followed by 2-hour perfusion with normal solution. Another 13 hearts were perfused with normal solution for 3 hours (Control).
Collagen content was 3.5 +/- 0.5% of ventricular dry weight in control group compared with 2.1 +/- 0.4% in DTNB group (decrease by 40%, p < 0.01). Scanning electron micrographs revealed loss of the delicate collagen network surrounding muscle fibers in DTNB treated hearts. Developed pressure at a fixed volume decreased to 86 +/- 17% of the baseline value after 3-hour perfusion in the control group, whereas in DTNB treated hearts developed pressure fell to 68 +/- 13% (p < 0.01). End-diastolic pressure was set at 5 mmHg at the beginning of the experiment and rose to 15 +/- 8 mmHg in control and 30 +/- 13 mmHg (p < 0.01) in the treated hearts. Concomitantly, wet-to-dry weight ratio increased from 5.63 +/- 0.26 in control to 6.07 +/- 0.11 (p < 0.05) in the DTNB treated hearts. A separate set of experiments on isolated myocytes excluded the possibility of a direct effect of DTNB on myocyte contractile function.
These data suggested that with 40% collagen disruption by DTNB there is a significant increase in tissue edema that results in a decrease in chamber capacitance; in addition, there is a significant decrease in systolic performance which reflects the combined effect of edema and loss of collagen.