CD24 induces localization of β1 integrin to lipid raft domains

• Published in: Biochemical and biophysical research communications Vol. 365; no. 1; pp. 35 - 41
• Main Authors: Runz, Steffen, Mierke, Claudia T., Joumaa, Safwan, Behrens, Jürgen, Fabry, Ben, Altevogt, Peter
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01-01-2008
• Subjects: CD24; Cell invasion; GPI-anchor; Integrins; Invasion; Lipid rafts; Membrane rafts; Motility; Transmigration; GPI-anchor; Transmigration; Motility; Cell invasion; Membrane rafts; Lipid rafts; CD24; Invasion; Integrins
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2007.10.139

The expression of the glycosyl phosphatidylinositol (GPI)-anchored protein CD24 correlates with poor prognosis in a variety of carcinomas. However, little is known about the cellular mechanisms of the CD24-mediated effects. In this study, we present evidence that CD24 affects the lateral localization of β1 integrin. Using stably CD24-transfected A125 and MDA-MB-435S carcinoma cells we show that CD24 augments β1-dependent cell motility and stimulates transmigration and invasion across a monolayer of endothelial cells. Furthermore, as demonstrated by sucrose density gradient centrifugation and Western Blot analysis, CD24 recruits β1 integrin into lipid raft domains. We suggest that CD24 acts as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins.