Surface plasmon resonance based colorimetric probe for vitamin B1 detection: Applications to bio-fluid analysis

• Published in: Case studies in chemical and environmental engineering Vol. 8; p. 100380
• Main Authors: Kondekar, Uttam R., Walekar, Laxman S., Pawar, Samadhan P., Mali, Mukund G., Anuse, Dilip D., Gardi, Chandrakant G., Deshmukh, Shamkumar, Anbhule, Prashant V., Kolekar, Govind B.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-12-2023; Elsevier
• Subjects: Absorption; Aggregation; Bio-fluids; Colour change; Gold nanoparticles; SPR; Thiamine; Aggregation; Absorption; Colour change; SPR; Gold nanoparticles; Bio-fluids; Thiamine
• ISSN: 2666-0164; 2666-0164
• DOI: 10.1016/j.cscee.2023.100380

This study reports simple analytical approach for thiamine (Vitamin B1) detection based on induced aggregation and alternation in colorimetric properties of gold nanoparticles (AuNPs), which was synthesized through citrate reduction approach. Furthermore, the citrate capped AuNPs are characterized by various analysing tools. The addition of thiamine persuades the aggregation of citrate-AuNPs and further leading to red to blue colour transition with decrease in absorbance intensity. The proposed method achieves good linearity with a correlation coefficient of 0.9843. By using our proposed strategy, thiamine was detected by unassisted vision as well as absorption spectroscopy. Under the most favorable condition method achieves good linear relationship between concentration range 0.01–0.8 μg mL−1 with limit of detection of 0.0067 μg mL−1. Under the premium condition, the method offers excellent selectivity towards thiamine detection in presence of different interfering species. Further practical applicability of the method was checked by using blood serum and urine sample via standard addition method. The obtained recoveries were acceptable in the range of 98.70–102.97% for added thiamine concentration. Thus, the proposed method may emerge as a target specific and highly sensitive tool towards thiamine detection.