New cytotoxic spirostane saponin and biflavonoid glycoside from the leaves of Acacia saligna (Labill.) H.L. Wendl

• Published in: Natural product research Vol. 28; no. 5; pp. 324 - 329
• Main Authors: Gedara, Sahar R., Galala, Amal A.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 04-03-2014
• Subjects: Acacia - chemistry; Acacia saligna; biflavonoid glycoside; Biflavonoids - chemistry; Biflavonoids - isolation & purification; Biflavonoids - pharmacology; cytotoxicity; Drug Screening Assays, Antitumor; Egypt; Fabaceae; flavonol glycosides; free radical scavenging activity; Glycosides - chemistry; Glycosides - isolation & purification; Glycosides - pharmacology; Hep G2 Cells; Humans; Mannosides - chemistry; Mannosides - isolation & purification; Nuclear Magnetic Resonance, Biomolecular; Plant Leaves - chemistry; Saponins - chemistry; Saponins - isolation & purification; Saponins - pharmacology; spirostane saponin; Spirostans - chemistry; Spirostans - isolation & purification; Spirostans - pharmacology; Egypt
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2013.863200

New naturally occurring spirostane saponin (25S)-5β-spirostan-3β-yl-3-O-β-d-xylopyranosyl(1 → 3)-O-β-d-xylopyranosyl(1 → 4)-β-d-galactopyranoside (6) and biflavonoid glycoside myricetin-3-O-rhamnoside (C7-O-C7) myricetin-3-O-rhamnoside (4) along with a series of known compounds erythrodiol (1), 3β-O-trans-p-coumaroyl-erythrodiol (2), quercetin-3-O-α-l-rhamnoside (3) and myricetin-3-O-α-l-rhamnoside (5) were separated from the leaves of Acacia saligna (Labill.), H.L. Wendl. Compounds 1 and 2 were separated for the first time from genus Acacia. The structures of compounds 1-6 were established on the basis of extensive 1D and 2D NMR spectroscopic techniques in combination with EI-MS and HR-ESI-MS. These compounds were screened for their antioxidant and cytotoxic activities against HEPG2 (liver cancer) cell line and significant results were obtained.