In vitro response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells exposed to 60Co at single fraction

• Published in: Brazilian archives of biology and technology Vol. 48; no. spe2; pp. 205 - 213
• Main Authors: Andrade, Lídia Maria(Department of Nuclear Engineering), Campos, Tarcisio Passos Ribeiro de(Department of Nuclear Engineering), Leite, M. F.(Department of Physiology and Biophysics), Góes, A. M.(Universidade Federal de Minas Gerais Department of Biochemistry and Immunology)
• Format: Journal Article
• Language: English
• Published: Tecpar 01-10-2005; Instituto de Tecnologia do Paraná (Tecpar)
• Subjects: Breast Cancer; MDAMB-231; PBMC and Gamma rays
• ISSN: 1516-8913; 1516-8913; 1678-4324
• DOI: 10.1590/S1516-89132005000700031

Radiotherapy using gamma rays is a common modality of breast cancer treatment. The aim of this research is to investigate the biological response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells (PBMC) exposed in vitro to 60 Co irradiation at a single fraction of 10 Gy, 25 Gy and 50 Gy doses at 136,4 cGy.min-1 rate. Cells were irradiated at room temperature by the Theratron 80 radiotherapy system. Biological response was evaluated through cellular viability using MTT assay and nucleus damages visualized by Propidium Iodide assay and electrophoresis agarose gel after gamma irradiation. Nucleus damages induced by 60Co irradiation were compared to damage caused by cell exposure to 10% methanol. The 50 Gy dose of irradiation did not stimulate nuclus damages at the same level as that affected by 10% methanol induction in the MDAMB-231. Further studies are necessary to understand these mechanisms in the MDAMB-231 human breast carcinoma cell line.
Radioterapia utilizando radiação gama é uma modalidade comum no tratamento do câncer de mama. A proposta deste estudo é investigar a resposta biológica in vitro da linhagem celular MDAMB-231 de câncer de mama humano e células do sangue periférico humano (PBMC) expostas à irradiação pelo Co60 em frações simples de 10Gy, 25Gy e 50Gy e 136,4cGy min-1 rate. As células foram irradiadas a temperatura ambiente usando o equipamento de radioterapia Theratron 80 radiotherapy system. A resposta biológica, após irradiação gama, foi avaliada através do ensaio do MTT para viabilidade celular e o do ensaio com Iodeto de Propídio para visualização do dano nuclear, além da eletroforese em gel de agarose. Os danos nucleares induzidos pelo Co60 foram comparados aos danos causados pela exposição das células à solução de metanol a 10%. Nós observamos que a dose de 50Gy não estimulou a mesma quantidade de danos nucleares que a solução de metanol a 10% nas células MDAMB-231. Maiores estudos são necessários para a compreensão destes mecanismos nas células de câncer de mama humano MDAMB-231.