Neuronal nitric oxide synthase (nNOS) expression in the epithelial neuroendocrine cell system and nerve fibers in the gill of the catfish, Heteropneustes fossilis

• Published in: Acta histochemica Vol. 101; no. 4; pp. 437 - 448
• Main Authors: Mauceri, Angela, Fasulo, Salvatore, Ainis, Luigi, Licata, Aurelio, Rita lauriano, Eugenia, Martfnez, Alfredo, Mayer, Bernd, Zaccone, Giacomo
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01-11-1999
• Subjects: Animals; catfish; Catfishes - metabolism; Endothelins - analysis; Epithelial Cells - enzymology; epithelial neuroendocrine cells; Fluorescent Antibody Technique, Indirect; gill; Gills - enzymology; Gills - innervation; Heteropneustes fossilis; immunocytochemistry; Immunoenzyme Techniques; nerve fibers; Nerve Fibers - enzymology; neuronal nitric oxide synthase; Neurosecretory Systems - cytology; Neurosecretory Systems - enzymology; Nitric Oxide Synthase - biosynthesis; Nitric Oxide Synthase Type I; gill; Heteropneustes fossilis; neuronal nitric oxide synthase; immunocytochemistry; epithelial neuroendocrine cells; nerve fibers; catfish
• ISSN: 0065-1281; 1618-0372
• DOI: 10.1016/S0065-1281(99)80044-0

We studied immunohistochemically the localization of neuronal nitric oxide synthase (nNOS) in gills of an Indian catfish species,
Heteropneustes fossilis. It is shown that most of the epithelial neuroendocrine cells that are present in gill filaments and lamellae stained positively. Co-localization of nNOS and endothelin was also shown in neuroendocrine cells. A dense plexus of nNOScontaining nerve fibers was present beneath the gill epithelium, associated with efferent filament arteries and the basal side of neuroendocrine cells. nNOS immunopositive neurons were not found in gill areas. nNOS immunopositive neuroendocrine cells appeared to differ from neuroepithelial cells in gills of various teleost species, which are considered as oxygen-sensitive receptors and are present in the distal halves of gill filaments. Other types of neuroendocrine cells have been identified previously in other areas of gills using antibodies to serotonin and endothelin peptides. These cell types are likely to be involved in chemical regulation of the physiology of gill cells. In relation to the function of the other cell types, our data on nNOS localization suggest that NO is a wide-spread transmitter in the gill of the Indian catfish.
It may play a role both in the local regulation of vascular tone and in inhibitory innervation of the gill.