Effects of epidermal growth factor on apo B mRNA levels and apo B accumulation in the media of primate hepatocytes in culture

• Published in: Biochemical and biophysical research communications Vol. 186; no. 1; p. 199
• Main Authors: Blake, W L, Vidmar, T J, Melchior, G W
• Format: Journal Article
• Language: English
• Published: United States 15-07-1992
• Subjects: Analysis of Variance; Animals; Apolipoprotein A-I - metabolism; Apolipoproteins B - genetics; Apolipoproteins B - metabolism; Cells, Cultured; Dose-Response Relationship, Drug; Epidermal Growth Factor - pharmacology; Kinetics; Liver - drug effects; Liver - metabolism; Macaca fascicularis; RNA, Messenger - genetics; RNA, Messenger - metabolism; Serum Albumin - metabolism; Time Factors
• ISSN: 0006-291X
• DOI: 10.1016/S0006-291X(05)80793-4

EGF has been shown to augment albumin and apolipoprotein A-I secretion by cynomolgus monkey hepatocytes in primary culture without stimulating cell division. This study was undertaken to determine what effect EGF had on apo B secretion by those hepatocytes. The results indicate that EGF (3 nM final concentration) severely inhibits the rate at which apo B accumulates in the culture medium of primate hepatocytes. That effect was evident within 48 hours of treatment, and by 72 hours the rate that apo B accumulated was less than half that of cells treated with a hormone-free medium. However, the apo B mRNA levels in the EGF-treated cells were more than double those of hepatocytes given the hormone-free medium. These data indicate that EGF has a potent effect on the rate at which apo B accumulates in the culture medium of primate hepatocytes and that the effect is independent of apo B gene expression.