Semen alterations and flow-citometry evaluation in patients with male accessory gland infections

• Published in: Journal of endocrinological investigation Vol. 35; no. 2; pp. 219 - 223
• Main Authors: La Vignera, S., Condorelli, R., D’Agata, R., Vicari, E., Calogero, A. E.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-02-2012
• Subjects: Adult; Bacterial Infections - complications; Bacterial Infections - epidemiology; Bacterial Infections - microbiology; Bacterial Infections - pathology; Case-Control Studies; Cells, Cultured; Endocrinology; Flow Cytometry - methods; Genital Diseases, Male - complications; Genital Diseases, Male - epidemiology; Genital Diseases, Male - microbiology; Genital Diseases, Male - pathology; Humans; Infertility, Male - epidemiology; Infertility, Male - etiology; Infertility, Male - microbiology; Infertility, Male - pathology; Male; Medicine; Medicine & Public Health; Metabolic Diseases; Microbiological Techniques; Original Article; Semen - cytology; Semen - microbiology; Semen - physiology; Semen Analysis - methods; Urinary Tract Infections - complications; Urinary Tract Infections - epidemiology; Urinary Tract Infections - microbiology; Urinary Tract Infections - pathology; Young Adult; sperm apoptosis; Chromatin compactness; DNA fragmentation; mitochondrial membrane potential
• ISSN: 0391-4097; 1720-8386
• DOI: 10.3275/7924

Male accessory gland infections (MAGI) represent a major cause of male infertility mainly through the secretory dysfunction of the prostate, seminal vesicles, and epididymis. This study was undertaken to evaluate conventional and non-conventional sperm parameters in these patients, therefore 150 patients with MAGI were selected. Each of them underwent to two sperm analyses and evaluation of DNA fragmentation mitochondrial membrane potential (MMP), phosphatidylserine (PS) externalization, chromatin compactness, by flow cytometry. Results showed that patients with MAGI had a lower sperm progressive motility (11.4 ±5.0 vs 34.0 ±7.0%), and percentage of normal forms (9.0 ±3.7 vs 33.0 ±13.0%) compared to controls, instead, these patients showed higher number of seminal white blood cells (2.2 ±1.0 vs 0.4 ±0.6 10
6
/ml). Patients with MAGI showed a higher number of spermatozoa with DNA fragmentation compared to controls (8.2 ±3.0 vs 1.0 ±1.0%). In addition, they have also a higher percentage of spermatozoa with low MMP (28.0 ±4.0 vs 2.0 ±2.0%). Patients with MAGI, showed a higher percentage of spermatozoa with PS externalization (8.0 ±4.0 vs 3.0 ±3.0%), an early sign of apoptosis, and lower percentage of viable spermatozoa (64.5 ±12.0 vs 88.0 ±10.0%). An increased percentage of spermatozoa with abnormal chromatin compactness (18.0 ±4.0 vs 5.0 ±3.0%) was found in patients with MAGI. In conclusion, patients with MAGI show alterations of conventional and biofunctional sperm parameters compared to controls. These results suggest to consider the flow cytometry evaluation among the diagnostic tools for male infertility.