An in situ Evaluation of Bioactives on the Morphology of Bleached Enamel

• Published in: The journal of contemporary dental practice Vol. 17; no. 3; pp. 192 - 197
• Main Authors: da Rosa Nogueira, Thiago, Alexandrino, Larissa Dias, de Lima Gomes, Yasmin do Socorro Batista, de Melo Alencar, Cristiane, Alves, Eliane Bemerguy, Silva, Cecy Martins
• Format: Journal Article
• Language: English
• Published: India 01-03-2016
• Subjects: Caseins - pharmacology; Dental Enamel - anatomy & histology; Dental Enamel - drug effects; Dentistry; Glass; Humans; Microscopy, Electron, Scanning; Surface Properties; Young Adult; Scanning electron microscopy; NANO-P; Rugosity analysis; Bioactive agents
• ISSN: 1526-3711; 1526-3711
• DOI: 10.5005/jp-journals-10024-1826

The aim of this study was to use surface rugosity analysis (Ra) and scanning electron microscopy (SEM) comparing effects of nano-hydroxyapatite (NANO), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), and NovaMin (NOVA) on enamel's human morphology bleached with 37.5% hydrogen peroxide.
Forty specimens (3 × 3 × 3 mm) were obtained from fully included third molars and four specimens were attached in the first molars of the volunteers. The POLA-positive control has only been bleached. Three experimental groups were bleached and treated with respective bioactive: NANO, CPP-ACP, and NovaMin. The Ra analyses were performed before and after the treatment using a rugosimeter. The obtained photomicrographs were analyzed using SEM (n = 3) by three examiners, and the study was double blind.
The Ra results were analyzed by one-way analysis of variance and Tukey's post hoc test (p < 0.05). All experimental groups showed significant differences of the others; however, the experimental groups were not significantly different from each other.
The enamel morphology of the bioactive-treated groups had more regular surfaces, than the others.