Widespread dissemination of extended-spectrum β-lactamase-producing, multidrug-resistant Escherichia coli in livestock and fishery products in Vietnam

• Published in: International journal of food contamination Vol. 2; no. 1; pp. 1 - 6
• Main Authors: Le, Ha Viet, Kawahara, Ryuji, Khong, Diep Thi, Tran, Hoa Thi, Nguyen, Thang Nam, Pham, Khai Ngoc, Jinnai, Michio, Kumeda, Yuko, Nakayama, Tatsuya, Ueda, Shuhei, Yamamoto, Yoshimasa
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 29-12-2015; Springer Nature B.V
• Subjects: Antibiotic resistance; Antibiotics; Bacteria; Chemistry; Chemistry and Materials Science; Data Article; Developing countries; Drug resistance; E coli; Escherichia coli; Fish; Fisheries; Fishery products; Food; Food contamination; Food contamination & poisoning; Food Science; Genotypes; LDCs; Livestock; Multidrug resistance; Phylogeny; Pork; Poultry; Public health; Rural areas; Vietnam; Extended-spectrum β-lactamase (ESBL); Livestock products; Antibiotic resistance
• ISSN: 2196-2804; 2196-2804
• DOI: 10.1186/s40550-015-0023-1

Background
Widespread dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria is a threat to public health. Since livestock products are possible reservoirs of ESBL-producing bacteria, food-borne dissemination of ESBL-producing bacteria and the characteristics of such organisms in food products should be assessed to evaluate potential sources of contamination.
Results
A total of 253 food samples from poultry, pork, shrimp, and fish were collected from local markets in a rural area of Vietnam from 2013 to 2014. ESBL-producing
Escherichia coli
were isolated from these samples, and their ESBL genotypes, phylogenetic groups, and antibiotic resistance profiles were assessed. Overall, a high percentage (68.4 %) of the food samples was contaminated with ESBL-producing
E. coli
, and samples from livestock and fishery products showed similar levels of contamination. The ESBL-producing
E. coli
isolated from the food samples harbored genes of the CTX-M-1, CTX-M-9, and TEM groups. Analysis of the antibiotic resistance profiles of the ESBL-producing
E. coli
isolates from the food samples showed a high degree of multidrug resistance. The prevalence of extensively multidrug-resistant ESBL-producing
E. coli
that were resistant to at least five antibiotic drug classes in poultry, pork, shrimp, and fish samples, was 92.1, 69, 56.5, and 62.5 %, respectively.
Conclusions
The results of this study confirmed the widespread dissemination of ESBL-producing
E. coli
in both livestock and fishery products from a rural area in Vietnam. The high prevalence of extensively multidrug-resistant ESBL-producing
E. coli
in food products highlights the importance of continuous monitoring of food products for the presence of these bacteria, particularly in underdeveloped countries.