cDNA sequences and organization of IgM heavy chain genes in two holostean fish
Immunoglobulin M heavy chain (μ) sequences of two holostean fish, the bowfin, Amia calva, and the longnose gar, Lepisosteus osseus, were amplified from spleen mRNA by RACE-PCR, cloned, and sequenced. Each μ chain showed the conserved four constant domain structure typical of a secreted μ chain. Sout...
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| Published in: | Developmental and comparative immunology Vol. 19; no. 2; pp. 153 - 164 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
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United States
Elsevier Ltd
01-03-1995
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| Abstract | Immunoglobulin M heavy chain (μ) sequences of two holostean fish, the bowfin,
Amia calva, and the longnose gar,
Lepisosteus osseus, were amplified from spleen mRNA by RACE-PCR, cloned, and sequenced. Each μ chain showed the conserved four constant domain structure typical of a secreted μ chain. Southern blot analyses with specific heavy chain variable (V
H) and constant (C
H) region probes suggest that both fish possess an IgH locus that resembles that of the teleosts, amphibians, and mammals in its organization. The overall sequence similarity of gar and bowfin μ chains was 60% and 48% at the nucleotide and amino acid levels, respectively, while similarity to the μ chains of teleosts and elasmobranchs was lower. The bowfin μ chain possesses a distinctive proline-rich sequence at the Cμ1/Cμ2 boundary; a shorter proline-rich sequence is present at this position in the gar μ chain. Both gar and bowfin show, in their Cμ4 sequences, motifs that could serve as cryptic splice donor sites for the production of mRNA encoding the membrane-bound form of the μ chains, and the bowfin also shows a potential cryptic splice donor site in the Cμ3 exon. |
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| AbstractList | Immunoglobulin M heavy chain (mu) sequences of two holostean fish, the bowfin, Amia calva, and the longnose gar, Lepisosteus osseus, were amplified from spleen mRNA by RACE-PCR, cloned, and sequenced. Each mu chain showed the conserved four constant domain structure typical of a secreted mu chain. Southern blot analyses with specific heavy chain variable (VH) and constant (CH) region probes suggest that both fish possess an IgH locus that resembles that of the teleosts, amphibians, and mammals in its organization. The overall sequence similarity of gar and bowfin mu chains was 60% and 48% at the nucleotide and amino acid levels, respectively, while similarity to the mu chains of teleosts and elasmobranchs was lower. The bowfin mu chain possesses a distinctive proline-rich sequence at the C mu 1/C mu 2 boundary; a shorter proline-rich sequence is present at this position in the gar mu chain. Both gar and bowfin show, in their C mu 4 sequences, motifs that could serve as cryptic splice donor sites for the production of mRNA encoding the membrane-bound form of the mu chains, and the bowfin also shows a potential cryptic splice donor site in the C mu 3 exon. Immunoglobulin M heavy chain (μ) sequences of two holostean fish, the bowfin, Amia calva, and the longnose gar, Lepisosteus osseus, were amplified from spleen mRNA by RACE-PCR, cloned, and sequenced. Each μ chain showed the conserved four constant domain structure typical of a secreted μ chain. Southern blot analyses with specific heavy chain variable (V H) and constant (C H) region probes suggest that both fish possess an IgH locus that resembles that of the teleosts, amphibians, and mammals in its organization. The overall sequence similarity of gar and bowfin μ chains was 60% and 48% at the nucleotide and amino acid levels, respectively, while similarity to the μ chains of teleosts and elasmobranchs was lower. The bowfin μ chain possesses a distinctive proline-rich sequence at the Cμ1/Cμ2 boundary; a shorter proline-rich sequence is present at this position in the gar μ chain. Both gar and bowfin show, in their Cμ4 sequences, motifs that could serve as cryptic splice donor sites for the production of mRNA encoding the membrane-bound form of the μ chains, and the bowfin also shows a potential cryptic splice donor site in the Cμ3 exon. |
| Author | Ravenstein, Eric van Clem, L.William Miller, Norman W. Warr, Gregory W. Middleton, Darlene L. Wilson, Melanie R. |
| Author_xml | – sequence: 1 givenname: Melanie R. surname: Wilson fullname: Wilson, Melanie R. organization: Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216-4505 U.S.A – sequence: 2 givenname: Eric van surname: Ravenstein fullname: Ravenstein, Eric van organization: Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216-4505 U.S.A – sequence: 3 givenname: Norman W. surname: Miller fullname: Miller, Norman W. organization: Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216-4505 U.S.A – sequence: 4 givenname: L.William surname: Clem fullname: Clem, L.William organization: Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216-4505 U.S.A – sequence: 5 givenname: Darlene L. surname: Middleton fullname: Middleton, Darlene L. organization: Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425-2211 U.S.A – sequence: 6 givenname: Gregory W. surname: Warr fullname: Warr, Gregory W. organization: Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425-2211 U.S.A |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7556802$$D View this record in MEDLINE/PubMed |
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| Keywords | cDNA sequence Gar Bowfin Immunoglobulin gene organization Holostean fish |
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| Snippet | Immunoglobulin M heavy chain (μ) sequences of two holostean fish, the bowfin,
Amia calva, and the longnose gar,
Lepisosteus osseus, were amplified from spleen... Immunoglobulin M heavy chain (mu) sequences of two holostean fish, the bowfin, Amia calva, and the longnose gar, Lepisosteus osseus, were amplified from spleen... |
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| SubjectTerms | Amino Acid Sequence Animals Base Sequence Bowfin cDNA sequence DNA, Complementary - isolation & purification Fishes - genetics Gar Holostean fish Immunoglobulin Constant Regions - genetics Immunoglobulin gene organization Immunoglobulin Heavy Chains - genetics Immunoglobulin M - genetics Immunoglobulin mu-Chains - genetics Molecular Sequence Data Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid |
| Title | cDNA sequences and organization of IgM heavy chain genes in two holostean fish |
| URI | https://dx.doi.org/10.1016/0145-305X(94)00063-L https://www.ncbi.nlm.nih.gov/pubmed/7556802 https://search.proquest.com/docview/77609324 |
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