Paraoxonase 1 activity in the sperm-rich portion of boar ejaculates is positively associated with sperm quality

• Published in: Animal reproduction Vol. 19; no. 3; p. e20220039
• Main Authors: Lucca, Matheus Schardong, Goularte, Karina Lemos, Rovani, Monique Tomazele, Schneider, Augusto, Gasperin, Bernardo Garziera, Lucia Júnior, Thomaz, Rossi, Carlos Augusto Rigon
• Format: Journal Article
• Language: English
• Published: Colégio Brasileiro de Reprodução Animal 2022
• Subjects: BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Original; DNA integrity; membrane integrity; spermatozoa kinetics; PON1
• ISSN: 1806-9614; 1984-3143; 1984-3143
• DOI: 10.1590/1984-3143-ar2022-0039

Associations of the activity of the paraoxonase 1 (PON1) enzyme with boar sperm quality still needs to be characterized, since boar ejaculates present distinct portions with differences in sperm concentration and quality. This study evaluated PON1 activity in the serum, in the distinct portions of boar ejaculates and estimated correlations with sperm quality parameters. Ejaculates and blood samples were collected from six boars for three weeks (two per week per boar;
n
= 36). Serum and post-spermatic portion PON1 activities were positively correlated (
P
= 0.01) but were both uncorrelated with the PON1 activity in the sperm-rich portion and in the whole ejaculate (
P
> 0.05). Differences in PON1 activity among boars were only observed in the sperm-rich portion of the ejaculate (
P
< 0.05). The PON1 activity in the serum and in the post-spermatic portion was generally negatively correlated with parameters of spermatozoa kinetics (
P
< 0.05). In the sperm-rich portion, PON1 activity was positively correlated with sperm concentration (
P
< 0.0001), curvilinear distance and velocity (both
P
< 0.05) and DNA integrity (
P
< 0.05), but negatively correlated with straightness and linearity (
P
< 0.05). Thus, boar ejaculates with increased PON1 activity in the sperm-rich portion may present increased concentration and spermatozoa with acceptable curvilinear velocity and distance and DNA integrity, which suggests that PON1 activity may be a biomarker for potential fertility.