Growth of serotypes I and II and variant strains of infectious bursal disease virus in Vero cells

• Published in: Avian diseases Vol. 32; no. 2; pp. 298 - 303
• Main Authors: Kibenge, F.S.B, Dhillon, A.S, Russell, R.G
• Format: Journal Article
• Language: English
• Published: United States American Association of Avian Pathologists, Inc 01-04-1988
• Subjects: Animals; AVIAN INFECTIOUS BURSITIS; CELL CULTURE; Cell culture techniques; Cell growth; Cell lines; Chick Embryo; CHICKENS; CULTIVO DE CELULAS; CULTURE DE CELLULES; Cultured cells; ENFERMEDAD DE GUMBORO; Fibroblasts; Infectious bursal disease virus; Infectious bursal disease virus - classification; Infectious bursal disease virus - growth & development; Infectious diseases; MALADIE DE GUMBORO; POLLO; POULET; Reoviridae - growth & development; Serotyping; Vero Cells; Viral diseases; Virus Cultivation; Viruses
• ISSN: 0005-2086; 1938-4351
• DOI: 10.2307/1590816

The growth of five strains of infectious bursal disease virus-three strains of serotype I (SAL, D-78, 2512), one of serotype II (OH), and one variant strain (Variant-A)-were compared in Vero and chicken embryo fibroblast (CEF) cell cultures in order to characterize the replication of different strains of IBDV in Vero cells. For all five virus strains, the latent period in Vero cells ranged from 12 to 18 hr, which was longer than the 4-to-6-hr latent period observed in CEF cultures for strains SAL, D-78, and OH. Virus strains SAL, D-78, and OH, which were examined in both Vero and CEF cultures, also had a more extensive maturation phase and higher yields of virus in Vero than in CEF cultures. Total titers of these viruses of 5.35 to 6.10 log10TCID50ml in CEFs occurred 24 to 30 hr postinoculation (PI), although the cytopathic effect (CPE) was not seen until 72 hr PI. By comparison, their total infectious virus titers of 6.85 to 8.35 log10TCID50ml in Vero cells occurred from 48 hr PI, coinciding with the appearance of CPE. The growth curve of Variant-A in Vero cells differed from the other viruses by showing steadily rising extracellular and cell-associated virus titers throughout the 72-hr observation period. Only very low titers of Variant-A were obtained in CEF cultures, and thus no growth curve in CEFs was performed. /// Se comparó el crecimiento de cinco cepas del virus de la enfermedad infecciosa de la bolsa en células Vero y en fibroblasto de embrión de pollo. Tres cepas pertenecían al serotipo I, una al serotipo II y la última fue una cepa clasificada como variante. El período latente en las células Vero fue de 12 a 18 horas, siendo mayor que el de 4 a 6 horas observado en el fibroblasto de embrión de pollo. Así mismo, se observó una fase de maduración más extensa y la produccón de virus fue mayor en las células Vero que en el fibroblasto. Los títulos totales del virus en fibroblasto fueron de 5.35 a 6.10 log10DICC50por ml y ocurrieron a las 24-30 horas después de la inoculación, aunque el efecto citopático no se observó hasta las 72 horas después de la inoculación. Los títulos totales de virus infeccioso en las células Vero fueron de 5.35 a 8.35 log10DICC50por ml y ocurrieron a las 48 horas después de la inoculación, coincidiendo con el efecto citopatogénico.