Molecular Analysis of Bacterial Community DNA in Sludge Undergoing Autothermal Thermophilic Aerobic Digestion (ATAD): Pitfalls and Improved Methodology to Enhance Diversity Recovery

Molecular analysis of the bacterial community structure associated with sludge processed by autothermal thermophilic aerobic digestion (ATAD), was performed using a number of extraction and amplification procedures which differed in yield, integrity, ability to amplify extracted templates and specif...

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Bibliographic Details

Published in:	Diversity (Basel) Vol. 2; no. 4; pp. 505 - 526
Main Authors:	Piterina, Anna V., Bartlett, John, Pembroke, J. Tony
Format:	Journal Article
Language:	English
Published:	Basel MDPI AG 01-04-2010
Subjects:	Aerobic digestion ATAD Chelation clone library Community structure Deoxyribonucleic acid DGGE DNA DNA extraction microbial community analysis PCR inhibition PCR optimisation Sludge Sludge digestion thermophilic sludge
Online Access:	Get full text
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