Rapid tissue diagnosis of herpes simplex infections in the newborn

The presence of intranuclear inclusions in tissues is considered to be consistent with viral infections. Further identification is done by viral cultures. In this study, the direct immunofluorescence technique was utilized to identify herpes simplex virus in the tissues. Positive fluorescence was se...

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Bibliographic Details
Published in:	Pediatric pathology Vol. 1; no. 3; p. 301
Main Authors:	Kapur, S, Chandra, R S
Format:	Journal Article
Language:	English
Published:	United States 1983
Subjects:	Brain - ultrastructure Encephalitis - diagnosis Encephalitis - etiology Encephalitis - pathology Fluorescent Antibody Technique Herpes Simplex - diagnosis Humans Inclusion Bodies, Viral - ultrastructure Infant, Newborn Microscopy, Electron Virus Cultivation
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Summary:	The presence of intranuclear inclusions in tissues is considered to be consistent with viral infections. Further identification is done by viral cultures. In this study, the direct immunofluorescence technique was utilized to identify herpes simplex virus in the tissues. Positive fluorescence was seen in 8 of 12 specimens. In all these specimens, diagnosis was further confirmed by electron microscopy, viral cultures, or both. Thus, the technique is a specific, sensitive method of diagnosis for herpes simplex infections. Rapidity and simplicity of the technique warrant regular use.
ISSN:	0277-0938
DOI:	10.3109/15513818309040667