The Diatom Nanofrustulum shiloi As a Promising Species in Modern Biotechnology

• Published in: Applied biochemistry and microbiology Vol. 60; no. 3; pp. 483 - 495
• Main Authors: Blaginina, A. A., Zheleznova, S. N., Miroshnichenko, E. S., Gevorgiz, R. G., Ryabushko, L. I.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-06-2024; Springer Nature B.V
• Subjects: Algae; Biochemistry; Biomass; Biomedical and Life Sciences; Biosynthesis; Biotechnology; Cell culture; Dry weight; Fatty acids; Fucoxanthin; Life Sciences; Medical Microbiology; Microbiology; New species; Polyunsaturated fatty acids; Pure culture; Scanning microscopy; Weight; Black Sea; productivity; diatom; fucoxanthin; intensive cultivation; polyunsaturated fatty acids
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683824603615

The results of studies of an intensive culture of a new species of bentoplanktonic diatom
N. shiloi
(Lee, Reimer et McEnery) Round, Hallsteinsen et Paasche 1999 isolated from the Black Sea are presented. Detailed descriptions are provided of the methods used to isolate the species into an algologically pure culture and its morphological and taxonomic features under light and electron scanning microscopy. The biochemical and production characteristics were also studied including the ability of the strain to accumulate fucoxanthin (
Fx
) and polyunsaturated fatty acids (PUFA) in laboratory conditions. During the exponential growth phase, the specific culture growth rate was µ = 0.8 1/day, and the maximum productivity
P
= 0.46 g dry weight/(L day). The accumulation of PUFAs in the biomass of
N. shiloi
reached 67.39 mg/g dry weight of algae. The
Fx
concentration in the biomass at the beginning of the stationary growth phase was 10 mg/g dry weight. The fairly high rate of
Fx
biosynthesis in microalgae cells, as well as the composition of fatty acids of the Black Sea strain, makes it possible to classify
N. shiloi
as a promising object in biotechnology.