Bioluminescent Toxicity Assay of Polyethylenimine-Based Sorbents

• Published in: Applied biochemistry and microbiology Vol. 57; no. 7; pp. 828 - 835
• Main Authors: Orlova, A. A., Aleskerova, L. E., Vasilieva, S. G., Morozov, A. S., Ismailov, A. D., Lobakova, E. S.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-12-2021; Springer Nature B.V
• Subjects: Animal migration; Biochemistry; Bioluminescence; Biomedical and Life Sciences; Control; Crosslinking; Diethylene glycol; Ethanol; Freezing; Immobilization; Life Sciences; Luminescence; Medical Microbiology; Metrology; Microbiology; Polyethyleneimine; Saline solutions; Scanning electron microscopy; Sorbents; Standardization; Toxicity; toxicity analysis; biotest; photobacteria; bioluminescence; polymer sorbents; polyethylenimine
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683821070061

The toxicity of polyethylenimine-based sorbents and their extracts was evaluated, and their effect on the bioluminescence of
Photobacterium phosphoreum
photobacteria was studied. These test bacteria are commonly used as objects to evaluate the toxicity of various materials. The analyzed materials were synthesized via polyethylenimine (PEI) cross-linking with diethylene glycol diglycidyl ether (DGDE) at mass contents of the latter of 1.9–120.0% with subsequent freezing. It was found that the degree of luminescence inhibition in
P. phosphoreum
cells depended on the PEI/DGDE ratio in the sorbent. Sorbents with a high DGDE content (60–120%) did not affect the cell luminescence activity, while those with a lower percentage of the cross-linker (0.9–30%) exhibited a pronounced inhibitory effect on luminescence of photobacteria according to the data obtained with the standard biotesting method. It was also established that the inhibitory effect of sorbents with a reduced DGDE percentage (<30%) in a phosphate buffer was significantly lower than that in salt solutions. Water and ethanol extracts of sorbents with a DGDE mass proportion of more than 15% did not significantly inhibit the luminescence of
P. phosphoreum
in 1 h of incubation. The immobilization of
P. phosphoreum
cells on the surface and internal parts of the studied sorbents was observed via scanning electron microscopy.