Microbispora cellulosiformans sp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold region

• Published in: Antonie van Leeuwenhoek Vol. 113; no. 12; pp. 2053 - 2062
• Main Authors: Gong, Xiujie, Xiang, Wensheng, Cao, Xu, Yu, Yang, Hao, Yubo, Li, Liang, Wang, Qiuju, Zou, Hongtao, Qian, Chunrong
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-12-2020; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Cardiolipin; Cell culture; Cellulase; Cold regions; Deoxyribonucleic acid; DNA; Fatty acids; Gene sequencing; Glycolipids; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Original Paper; Phosphatidylethanolamine; Phosphatidylinositol; Phospholipids; Phylogeny; Plant Sciences; rRNA 16S; Soil Science & Conservation; Soils; Strains (organisms); Ubiquinone; Polyphasic taxonomy; sp. nov; Cellulase activity; 16S rRNA gene
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-020-01477-4

A novel cellulase-producing actinomycete strain Gxj-6
T
, isolated from soil in the cold region (Heihe city, Heilongjiang province, the northernmost part of China), subjected to a taxonomic study using a polyphasic approach. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain Gxj-6
T
fell within the clade comprising the type strains of species of the genus
Microbispora
. 16S rRNA gene sequence similarity studies exhibited that species Gxj-6
T
was most closely related to
Microbispora bryophytorum
NEAU-TX2-2
T
(99.45%),
Microbispora fusca
NEAU-HEGS1-5
T
(99.41%),
Microbispora camponoti
2C-HV3
T
(99.31%) and
Microbispora rosea
subsp.
rosea
JCM 3006
T
(98.68%). Organism Gxj-6
T
contained MK-9(H
2
) as the predominant ubiquinone and C
18:0
10-methyl as the major fatty acid. The major polar lipids of culture Gxj-6
T
were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, three unidentified phospholipids, two unidentified glycolipids and two unidentified lipids. The DNA G+C content of strain Gxj-6
T
was 71.25 mol%. The morphological and chemotaxonomic properties of the strain are also consistent with those members of the genus
Microbispora
. Combinated with the lower DNA–DNA relatedness values, phenotypic properties, physiology and biochemistry distinctiveness with other recognized species strains, revealed that strain Gxj-6
T
is separated from other phylogenetically closely species of the genus
Microbispora
. Therefore, strain Gxj-6
T
is considered to represent a novel species of the genus
Microbispora
, for which the name
Microbispora cellulosiformans
sp. nov. is proposed. The type strain is Gxj-6
T
(= CGMCC 4.7605
T
 = DSM 109712
T
).