Genetic diversity of Barbary fig (Opuntia ficus-indica) collection in Greece with ISSR molecular markers
Barbary fig (Opuntia ficus indica) could be an economically important species as it could be an alternative crop extremely tolerant to dry condition and water deficiency. Moreover, it could be used in alternative sustainable cultivation systems and landscape conservation. In this work, we report the...
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| Published in: | Plant gene Vol. 2; pp. 29 - 33 |
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| Abstract | Barbary fig (Opuntia ficus indica) could be an economically important species as it could be an alternative crop extremely tolerant to dry condition and water deficiency. Moreover, it could be used in alternative sustainable cultivation systems and landscape conservation. In this work, we report the analysis of the genetic diversity of Greek Barbary fig genotypes using ISSR molecular markers. Six primers were screened to assess their ability to detect polymorphisms within twenty-two Barbary fig accessions and generated 57 markers (bands), with an average of 9.5 markers per primer. The percentage of polymorphic bands (50.21%) and the resolving power (RP) (28.85) showed the efficiency of the used primers. Mean values for GD (gene diversity) and I (Shannon index) were found as 0.215 and 0.355, respectively. The revealed ISSR markers allow distinguishing all accessions analyzed except for one case. UPGMA dendrogram and PCoA (Principal Coordinate Analysis) were performed to access patterns of diversity among genotypes. The high genetic diversity existing in the Greek germplasm suggests that it would be beneficial to utilize this pool in Barbary fig breeding programs and germplasm management activities.
•Greek Barbary fig germplasm genotyping•Use of ISSR molecular markers in Barbary fig genotypes•Molecular characterization of Greek germplasm collection•ISSR markers an effective marker system for evaluation of Barbary genetic diversity |
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| AbstractList | Barbary fig (Opuntia ficus indica) could be an economically important species as it could be an alternative crop extremely tolerant to dry condition and water deficiency. Moreover, it could be used in alternative sustainable cultivation systems and landscape conservation. In this work, we report the analysis of the genetic diversity of Greek Barbary fig genotypes using ISSR molecular markers. Six primers were screened to assess their ability to detect polymorphisms within twenty-two Barbary fig accessions and generated 57 markers (bands), with an average of 9.5 markers per primer. The percentage of polymorphic bands (50.21%) and the resolving power (RP) (28.85) showed the efficiency of the used primers. Mean values for GD (gene diversity) and I (Shannon index) were found as 0.215 and 0.355, respectively. The revealed ISSR markers allow distinguishing all accessions analyzed except for one case. UPGMA dendrogram and PCoA (Principal Coordinate Analysis) were performed to access patterns of diversity among genotypes. The high genetic diversity existing in the Greek germplasm suggests that it would be beneficial to utilize this pool in Barbary fig breeding programs and germplasm management activities.
•Greek Barbary fig germplasm genotyping•Use of ISSR molecular markers in Barbary fig genotypes•Molecular characterization of Greek germplasm collection•ISSR markers an effective marker system for evaluation of Barbary genetic diversity |
| Author | Ganopoulos, Ioannis Kavroulakis, Nektarios Kalivas, Apostolos Xanthopoulou, Aliki Mastrogianni, Anna Koubouris, Georgios Madesis, Panagiotis |
| Author_xml | – sequence: 1 givenname: Ioannis surname: Ganopoulos fullname: Ganopoulos, Ioannis organization: Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki 57001, Greece – sequence: 2 givenname: Apostolos surname: Kalivas fullname: Kalivas, Apostolos organization: Cotton and Industrial Plants Institute, Hellenic Agricultural Organization “Demeter”, Thermi, Thessaloniki 57001, Greece – sequence: 3 givenname: Nektarios surname: Kavroulakis fullname: Kavroulakis, Nektarios organization: Institute of Olive Tree and Subtropical Plants of Chania, Hellenic Agricultural Organization “Demeter”, Agrokipio, 73100 Chania, Greece – sequence: 4 givenname: Aliki surname: Xanthopoulou fullname: Xanthopoulou, Aliki organization: Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki 57001, Greece – sequence: 5 givenname: Anna surname: Mastrogianni fullname: Mastrogianni, Anna organization: Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki 57001, Greece – sequence: 6 givenname: Georgios surname: Koubouris fullname: Koubouris, Georgios organization: Department of Botany, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece – sequence: 7 givenname: Panagiotis surname: Madesis fullname: Madesis, Panagiotis email: pmadsesis@certh.gr organization: Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki 57001, Greece |
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| Cites_doi | 10.1093/molbev/msm092 10.1007/978-1-4684-9063-3_14 10.1046/j.1365-294x.1998.00425.x 10.2307/1006195 10.1073/pnas.76.10.5269 10.3732/ajb.91.11.1915 10.1007/s10681-011-0416-z 10.1099/00207713-51-3-731 10.1007/s00606-010-0351-9 10.1002/mnfr.200400071 10.1016/j.scienta.2007.02.009 10.1073/pnas.70.12.3321 10.1016/S0168-9452(03)00321-2 10.1016/j.scienta.2013.04.015 10.4238/2011.November.4.7 10.1111/j.1471-8286.2005.01155.x 10.1016/j.jaridenv.2004.06.003 10.1007/s001220051046 |
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| Keywords | Opuntia ficus indica Genetic diversity Greek germplasm ISSR markers |
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| Title | Genetic diversity of Barbary fig (Opuntia ficus-indica) collection in Greece with ISSR molecular markers |
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