Antibiotic resistance in the invasive bacteria Escherichia coli

• Published in: Central European journal of public health Vol. 30; no. Supplement; pp. S75 - S80
• Main Authors: Lovayová, Viera, Čurová, Katarína, Hrabovský, Vladimír, Nagyová, Mária, Siegfried, Leonard, Toporová, Annamaria, Rimárová, Kvetoslava, Andraščíková, Štefánia
• Format: Journal Article
• Language: English
• Published: Prague National Institute of Public Health 01-06-2022
• Subjects: Antibiotic resistance; Antibiotics; Antiinfectives and antibacterials; Antimicrobial agents; Bacteria; Clinical isolates; Coliforms; Departments; E coli; Enzymes; Epidemiology; Genes; Hospitals; Infectious diseases; Ions; Software; β Lactamase; Asia; Europe
• ISSN: 1210-7778; 1803-1048
• DOI: 10.21101/cejph.a7384

Objectives: The beta-lactamases with extended spectrum of activity (ESBL) are medically one of the most important group of enzymes. Another group of beta-lactamases representing of Enterobacteriaceae is group of the AmpC-type cephalosporinases. The presented study provides identification and determination of the spectrum of resistance against different and clinically used antimicrobial drugs in the clinical isolates of Escherichia coli. Methods: These isolates had origin in different departments of the L. Pasteur University Hospital in Košice. The goal was the detection of betalactamase production with extended-spectrum effect and testing of AmpC-type cephalosporinases by several phenotypic tests in clinical isolates. MALDI-TOF MS analysis was performed on a Microflex MALDI Biotyper. Samples were positively tested for ESBL with the use of the disc diffusion method. PCR were performed with a series of primers designed for the detection of Ambler class A, B and C beta-lactamase genes. Results: For all 485 isolates, we determined the production of ESBL, which we detected in 166 E. coli isolates, which represents a 34.2% prevalence of ESBL production. It is clear from the results that the prevalence of ESBL-producing E. coli out of the total number of E. coli investigated reached 34.2%. In the monitored period, we confirmed at least one resistance gene from 485 E. coli in 188 positive isolates. Conclusions: We describe a complex ESBL epidemiology. The study revealed a high rate of ESBL-producing E. coli isolates; blaTEM and blaSHV enzymes dominated in ESBL-positive E. coli isolates in the L. Pasteur University Hospital in Košice.