Insulin-like growth factor binding protein 1 and human embryonic development during 6 -10 gestational weeks

• Published in: Chinese medical journal Vol. 117; no. 4; pp. 488 - 491
• Main Author: 方群 王艳霞 周祎
• Format: Journal Article
• Language: English
• Published: China Fetal Medicine Center, Department of Obstetrics and Gynecology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China 01-04-2004
• Subjects: Adult; Decidua - chemistry; Embryonic and Fetal Development; Female; Gestational Age; Humans; Insulin-Like Growth Factor Binding Protein 1 - analysis; Insulin-Like Growth Factor Binding Protein 1 - blood; Insulin-Like Growth Factor Binding Protein 1 - genetics; Pregnancy; RNA, Messenger - analysis; 临床意义; 妊娠期; 胚胎发育; 胰岛素样生长因子; 酶联免疫吸附试验; embryology; insulin-like growth-factor binding protein 1; pregnancy
• ISSN: 0366-6999; 2542-5641

Background Insulin-like growth factor binding protein-1 (IGFBP-1), which is a carrier of Insulin-like growth factors (IGFs) regulates the fetal development by working as an active factor controlling the combination of IGFs with their receptors. This study was designed to investigate the relationship between IGFBP-1 and human embryonic development during weeks 6 -10 of gestation.Methods A total of 44 pregnant women with singleton pregnancy were divided into two groups, one with abnormal embryo development (n=32) and the other with normal embryo development (n=12).Enzyme-linked immunosorbent assay (ELISA) was employed to detect IGFBP-1 levels in maternal serum and decidual tissue. The expression of IGFBP-1 mRNA in deciduas was examined by reverse transcription polymerase chain reaction (RT-PCR) technique.Results The level of IGFBP-1 protein in maternal serum was significantly higher in the abnormal group [(125.36±47.93)μg/ml] than in the normal group [(70.72±21.21 )μg/ml]. Both of IGFBP-1 and IGFBP-1 mRNA in deciduas were higher in abnormal group [ (1.60 ± 1.39) μg/ml and 1.66±1.64, respectively ] than in the normal group [ (0.35±0.23)μg/ml and 0.40±0.20,respectively]. The level of IGFBP-1 in maternal serum was positively correlated with IGFBP-1 mRNA(r=0.90, P<0.05) and IGFBP-1 protein (r=0.92, P<0. 05) in decidual tissue.Conclusions During weeks 6 -10 of gestation, abnormal embryonic development is correlated with elevated IGFBP-1. The level of IGFBP-1 in maternal serum is related to the concentrations of IGFBP-1 mRNA and IGFBP-1 in decidual tissue. The IGFBP-1 level in maternal serum may be used as a predictive marker to evaluate embryonic development.