Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method

• Published in: Chemical research in Chinese universities Vol. 31; no. 6; pp. 914 - 918
• Main Authors: Ma, Yinghui, Liu, Yuanyuan, Wei, Mengying, Song, Fengrui, Liu, Zhongying, Pi, Zifeng
• Format: Journal Article
• Language: English
• Published: Changchun Jilin University and The Editorial Department of Chemical Research in Chinese Universities 01-12-2015; College of Pharmacy, Jilin University, Changchun 130021, P.R.China%Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun 130022, P.R.China
• Subjects: Analytical Chemistry; Caco-2细胞; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; HPLC-ESI-MS; Inorganic Chemistry; Organic Chemistry; Physical Chemistry; 人参皂苷Re; 细胞模型; 肠道; 藜芦; 运输; 配伍; High performance liquid chromatography-electrospray ionization-mass spectroscopy(HPLC-ESI-MS); Transport; Caco-2 cell monolayer model; Ginsenoside; Veratrum nigrum; Panax ginseng
• ISSN: 1005-9040; 2210-3171
• DOI: 10.1007/s40242-015-5207-y

The Caco-2 cells have been recognized as effective tools to be applied to imitating the drug absorption in human intestine for the transport of drug. Herein, Caco-2 cell monolayer model was used to study the transport of the ginsenoside compatibility with Veratrum nigrum in different proportions. A specific high performance liquid chro- matography-electrospray ionization-mass spectrometry（HPLC-ESI-MS） method was developed for the semiquantita- tive determination of ginsenoside in intestinal transport with Dioscin as an internal standard. For the Caco-2 model constructed, two influencing factors were investigated, including time and concentration. The results suggest that the absorption of ginsenoside Re, Rgl, Rbl, Rc, Rb2 and Rd are time- and concentration-dependent and the excretions of Rbl, Rc, Rb2 and Rd have a relatronship with some transport proteins. The bioavailability of the ginsenosides has reduced compared to the single Panax ginseng extract when compatibility with a certain amount of Veratrum nigrum.