Antimicrobial Activity of the Chitosan Extracted from Metapenaeus stebbingi Shell Wastes

• Published in: Journal of polymers and the environment Vol. 20; no. 2; pp. 431 - 437
• Main Authors: Küçükgülmez, Aygül, Gülnaz, Osman, Celik, Mehmet, Yanar, Yasemen, Eslem Kadak, A., Gerçek, Gözde
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-06-2012; Springer Nature B.V
• Subjects: Antimicrobial agents; Bacteria; Biopolymers; Chemistry; Chemistry and Materials Science; Chitosan; Disks; Dissolution; E coli; Environmental Chemistry; Environmental Engineering/Biotechnology; Escherichia coli; Hydrochloric acid; Industrial Chemistry/Chemical Engineering; Manufacturing engineering; Materials Science; Microorganisms; Original Paper; Polymer Sciences; Pseudomonas fluorescens; Shellfish; Shells; Waste materials; shell; Antimicrobial activity; Chitosan
• ISSN: 1566-2543; 1572-8919; 1572-8900
• DOI: 10.1007/s10924-011-0395-0

In the study, chitosan chemically extracted from
Metapenaeus stebbingi
shells obtained from shrimp processing factories and commercial chitosan were used as antimicrobial materials. Antimicrobial activities of the chitosans dissolved in acetic, lactic, formic and hydrochloric acid at different concentrations (1.00, 0.50, 0.25, 0.10 and 0.05%) were tested in vitro by using the disk diffusion method with standard microorganisms (
Pseudomonas putida
,
Pseudomonas fluorescens, Vibrio parahaemolyticus, Staphylococcus epidermidis
,
Staphylococcus aureus, Escherichia coli
1
, Escherichia coli
2,
Listeria monocytogenes, Enterecoccus faecalis, Aeromonas caviae
)
.
On the other hand, gentamicin was used as positive control. Antimicrobial test results indicated that chitosan demonstrated different effects depending on the chitosan concentration, solvent material and bacteria type. In the present study, both the extracted and commercial chitosans were observed to have antimicrobial effects on nearly all types of the bacteria.