Non-steroidal anti-inflammatory agents, Part 20. Method for testing non-steroidal anti-inflammatories: the modified hen's egg chorioallantoic membrane test (HET-CAM test) compared to other procedures

• Published in: Archiv der Pharmazie (Weinheim) Vol. 329; no. 6; p. 301
• Main Authors: Dannhardt, G, Kreher, M, Nowe, U, Pies, A
• Format: Journal Article
• Language: English
• Published: Germany 1996
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal - chemistry; Anti-Inflammatory Agents, Non-Steroidal - metabolism; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Anti-Inflammatory Agents, Non-Steroidal - toxicity; Chickens; Chorion - metabolism; Diffusion; Dose-Response Relationship, Drug; Eggs; Molecular Structure; Time Factors; Toxicity Tests - methods
• ISSN: 0365-6233
• DOI: 10.1002/ardp.19963290606

The delay of onset of irritation phenomena at the chorioallantoic membrane of incubated hen's eggs, a parameter for anti-inflammatory activity, was determined for the pharmaceutical substances diclofenac, flufenamic acid, ibuprofen, indomethacin, ketoprofen, piroxicam, phenylbutazone, salicylic acid, and sodium salicylate. Alongside questions relating to the dose-effect ratio, metabolisation, recovery, and diffusion of the substances to their site of action were investigated. The reproducibility of the procedure and its selectivity with regard to substances with a different mechanism of action is proven. The method allows classification of the substances according to their anti-inflammatory potency. However, correlation with the results of enzyme inhibition or in vivo results is only possible to a limited extent.