Genomics and Proteomic Techniques

• Published in: Methods in enzymology Vol. 603; p. 221
• Main Author: Pan, Jonathan Z
• Format: Journal Article
• Language: English
• Published: United States 2018
• Subjects: Anesthesia, General; Anesthetics, Inhalation - pharmacokinetics; Anesthetics, Inhalation - pharmacology; Animals; Carbon Radioisotopes; Cerebral Cortex - cytology; Cerebral Cortex - drug effects; Cerebral Cortex - metabolism; Electrophoresis, Gel, Two-Dimensional - methods; Fetus; Gene Regulatory Networks; Halothane - pharmacokinetics; Humans; Isoflurane - pharmacokinetics; Mice; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; Primary Cell Culture; Protein Binding; Protein Biosynthesis; Protein Interaction Mapping - statistics & numerical data; Proteogenomics - instrumentation; Proteogenomics - methods; Rats; Real-Time Polymerase Chain Reaction - methods; Sevoflurane - pharmacokinetics; Staining and Labeling - methods; Transcription, Genetic; DNA microarray; Pathway analysis; Differential in-gel electrophoresis; Genomics; Network; Proteomics; Statistics; Inhaled anesthetic
• ISSN: 1557-7988
• DOI: 10.1016/bs.mie.2018.02.008

Although general anesthesia induced by inhaled anesthetics produces definitive phenotypes (e.g., loss of mobility, amnesia, analgesia), the underlying targets of these drugs are still not clear. Genomics and proteomic techniques are discussed for measurement of global transcriptional and translational changes after inhaled anesthetic exposures. The current discussion focuses primarily on the genomic and proteomic technical methodology. We also include a discussion of network and pathway analyses for data interpretation after identification of the targets.