Modulation of nitric oxide production by tetracyclines and chemically modified tetracyclines

• Published in: Advances in dental research Vol. 12; no. 2; p. 126
• Main Authors: Cillari, E, Milano, S, D'Agostino, P, Di Bella, G, La Rosa, M, Barbera, C, Ferlazzo, V, Cammarata, G, Grimaudo, S, Tolomeo, M, Feo, S
• Format: Journal Article
• Language: English
• Published: United States 01-11-1998
• Subjects: Animals; Apoptosis - drug effects; Cell Line; Dexamethasone - pharmacology; Dose-Response Relationship, Drug; Lipopolysaccharides - pharmacology; Macrophages - drug effects; Macrophages - metabolism; Macrophages - physiology; Mice; Nitric Oxide - biosynthesis; Nitric Oxide Synthase - antagonists & inhibitors; Nitric Oxide Synthase - biosynthesis; omega-N-Methylarginine - pharmacology; Tetracyclines - chemistry; Tetracyclines - pharmacology
• ISSN: 0895-9374
• DOI: 10.1177/08959374980120010701

Chemically modified tetracyclines (CMTs) dose-dependently decreased inducible nitric oxide synthase (iNOS) and, consequently, nitric oxide (NO) formation by the lipopolysaccharide (LPS)-stimulated J774 line. The inhibitory effect was due to a specific reduction in the iNOS protein content in the cells, as attested by Western blot analysis and by the inhibition of iNOS mRNA accumulation. Furthermore, CMTs cause a dose-dependent increase in cell death in the J774 line mediated by the NO-independent apoptotic mechanism.