Evaluation of antioxidant, antimicrobial, enzyme inhibition activity, and cell viability capacity of Hypericum heterophyllum vent., an endemic species in Turkey's Flora

• Published in: Journal of molecular structure Vol. 1307; p. 137908
• Main Authors: Eruygur, Nuraniye, Uçar, Esra, Tüzün, Burak, Ataş, Mehmet, İnanır, Merve, Demirbaş, Ahmet, Bal, Halil, Şenkal, Belgin Coşge, Uskutoğlu, Tansu
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05-07-2024
• Subjects: Biological activity; Extract; Flowering; Hypericum; In silico; Flowering; In silico; Extract; Hypericum; Biological activity
• ISSN: 0022-2860; 1872-8014
• DOI: 10.1016/j.molstruc.2024.137908

•The aim of this study was to assess the macro and micro substances, antioxidant, antimicrobial, enzyme inhibition activity, and cell viability capacity of H. heterophyllum extracts, an endemic species in the flora of turkey.•Methanolic extract of H. heterophyllum was utilized in the study, with plants collected at the beginning and full flowering stages.•H. heterophyllum extracts exhibited significant pharmacological activities, with higher activity observed during the beginning of the flowering period.•The activities of the chemicals contained in H. heterophylum extracts against various proteins were compared, and ADME/T analysis was conducted on molecules exhibiting high activity.
The aim of this study was determined the antioxidant, antimicrobial, enzyme inhibition activity, and cell viability capacity of H. heterophyllum (endemic species in the Flora of Turkey) extracts. H. heterophyllum methanolic extract was used in this study. H. heterophyllum plants collected at the beginning of flowering and during full flowering. P content was determined with calorimetrically in spectrophotometer. K, CA, Mg, Fe, Mn, Zn and Cu was determined with Atomic Absorption and N concentration were determined according to the Kjeldahl distillation method. H. heterophyllum (endemic species in the Flora of Turkey) extract was evaluated for its antioxidant (DPPH, ABTS, total phenol and flavonoid content), antimicrobial (four bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus) and two Candida species (Candida albicans and Candida tropicalis)), enzyme inhibition activity (ɑ-glucosidase, ɑ-amylase, acetylcholinesterase, butyrylcholinesterase, tyrosinase), and cell viability (XTT assay) capacity. Extracts from the beginning of flowering period showed stronger biological activity. It has been determined that the extracts have antioxidant, antibacterial (against Staphylococcus aureus) anticancer activity (on breast cancer cells), and inhibition effects of BChE and alpha-glucosidase. It has been determined that the extracts obtained from H. heterophylum have important pharmacological activities, and the plant exhibits higher activity in the BF period. Hypericum heterophyllum Vent. The activities of the chemicals contained in it against various proteins that are α-gly enzyme (PDB ID: 1R47), AChE enzyme (PDB ID: 4M0E), BChE enzyme (PDB ID: 5NN0), alpha-Amylase (PDB ID: 3BAJ), breast cancer protein (PDB ID: 1A52 and 1JNX), were compared. ADME/T analysis of molecules with high activity was performed.