ABNOH‐Linked Nucleotides and DNA for Bioconjugation and Cross‐linking with Tryptophan‐Containing Peptides and Proteins

Reactive N‐hydroxy‐9‐azabicyclo[3.3.1]nonane (ABNOH) linked 2′‐deoxyuridine 5′‐O‐mono‐ and triphosphates were synthesized through a CuAAC reaction of ABNOH‐PEG4‐N3 with 5‐ethynyl‐dUMP or ‐dUTP. The modified triphosphate was used as substrate for enzymatic synthesis of modified DNA probes with KOD XL...

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Bibliographic Details
Published in:	Chemistry : a European journal Vol. 30; no. 49; pp. e202402151 - n/a
Main Authors:	Spampinato, Ambra, Leone, Denise‐Liu', Pohl, Radek, Tarábek, Ján, Šoltysová, Markéta, Polák, Marek, Kádek, Alan, Sýkorová, Veronika, Řezáčová, Pavlína, Hocek, Michal
Format:	Journal Article
Language:	English
Published:	Germany Wiley Subscription Services, Inc 02-09-2024
Subjects:	Bioconjugations Chemical synthesis Conjugates Cross-Linking Reagents - chemistry Crosslinking Deoxyribonucleic acid DNA DNA - chemistry DNA biosynthesis DNA polymerase DNA probes DNA-directed DNA polymerase DNA-Directed DNA Polymerase - chemistry DNA-Directed DNA Polymerase - metabolism Enzymatic synthesis N-oxyl radicals Nucleotides Nucleotides - chemistry Oligonucleotides Peptides Peptides - chemistry Proteins Proteins - chemistry Sodium nitrite Substrates Tryptophan Tryptophan - chemistry Nucleotides Peptides N-oxyl radicals Oligonucleotides Bioconjugations
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