Testusterol, a new sterol of the sponge species Xestospongia testudinaria from Phu Quoc island, Vietnam

• Published in: Natural product research pp. 1 - 9
• Main Authors: Vu Luu, Phuong, Minh Nguyen, Hien, Minh Phan, Phuc, Duy Vo, An, Ton-Nu, Huong Lien
• Format: Journal Article
• Language: English
• Published: England 11-04-2024
• Subjects: Vietnam; anti-microorganisms; Xestospongia testudinaria; cytotoxicity; testusterol
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2024.2340757

A new sterol, named testusterol (
), and five known compounds (
) were isolated from the
-hexane and dichloromethane extracts of the sponge species
. Their chemical structures were elucidated based on extensive spectroscopic analyses (1D, 2D NMR, ESIMS and HRESIMS) and comparison with published data. The results of
test (utilizing brine shrimp
LEACH) showed that three extracts ethanol, dichloromethane, and ethanol/water, significantly inhibited
with LC
values ranging from 6.09 to 16.83 µg/mL. Remarkably, the new compound
exhibited potent inhibition against both Gram-positive (
), and Gram-negative (
) bacteria species, with IC
values of less than 12.0 nM and MIC ranging from 4.70 to 75.23 nM as determined by the broth-microdilution assay.