Fabrication and mechanical characterization of hydrogel-based 3D cell-like structures
In this article, we demonstrate the fabrication of 3D cell-like structures using a femtosecond laser-based two-photon polymerization technique. By employing poly(ethylene glycol) diacrylate monomers as a precursor solution, we fabricate 3D hemispheres that resemble morphological and biomechanical ch...
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| Published in: | Optics express Vol. 31; no. 18; p. 29174 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
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28-08-2023
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| Abstract | In this article, we demonstrate the fabrication of 3D cell-like structures using a femtosecond laser-based two-photon polymerization technique. By employing poly(ethylene glycol) diacrylate monomers as a precursor solution, we fabricate 3D hemispheres that resemble morphological and biomechanical characteristics of natural cells. We employ an optical tweezers-based microrheology technique to measure the viscoelastic properties of the precursor solutions inside and outside the structures. In addition, we demonstrate the interchangeability of the precursor solution within fabricated structures without impairing the microstructures. The combination of two-photon polymerization and microrheological measurements by optical tweezers demonstrated here represents a powerful toolbox for future investigations into cell mimic and artificial cell studies. |
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| AbstractList | In this article, we demonstrate the fabrication of 3D cell-like structures using a femtosecond laser-based two-photon polymerization technique. By employing poly(ethylene glycol) diacrylate monomers as a precursor solution, we fabricate 3D hemispheres that resemble morphological and biomechanical characteristics of natural cells. We employ an optical tweezers-based microrheology technique to measure the viscoelastic properties of the precursor solutions inside and outside the structures. In addition, we demonstrate the interchangeability of the precursor solution within fabricated structures without impairing the microstructures. The combination of two-photon polymerization and microrheological measurements by optical tweezers demonstrated here represents a powerful toolbox for future investigations into cell mimic and artificial cell studies. In this article, we demonstrate the fabrication of 3D cell-like structures using a femtosecond laser-based two-photon polymerization technique. By employing poly(ethylene glycol) diacrylate monomers as a precursor solution, we fabricate 3D hemispheres that resemble morphological and biomechanical characteristics of natural cells. We employ an optical tweezers-based microrheology technique to measure the viscoelastic properties of the precursor solutions inside and outside the structures. In addition, we demonstrate the interchangeability of the precursor solution within fabricated structures without impairing the microstructures. The combination of two-photon polymerization and microrheological measurements by optical tweezers demonstrated here represents a powerful toolbox for future investigations into cell mimic and artificial cell studies.In this article, we demonstrate the fabrication of 3D cell-like structures using a femtosecond laser-based two-photon polymerization technique. By employing poly(ethylene glycol) diacrylate monomers as a precursor solution, we fabricate 3D hemispheres that resemble morphological and biomechanical characteristics of natural cells. We employ an optical tweezers-based microrheology technique to measure the viscoelastic properties of the precursor solutions inside and outside the structures. In addition, we demonstrate the interchangeability of the precursor solution within fabricated structures without impairing the microstructures. The combination of two-photon polymerization and microrheological measurements by optical tweezers demonstrated here represents a powerful toolbox for future investigations into cell mimic and artificial cell studies. |
| Author | Dzikonski, Dustin Imbrock, Jörg Kumar, Randhir Bekker, Elena Denz, Cornelia Vitali, Valerio Vornhusen, Robert |
| Author_xml | – sequence: 1 givenname: Randhir orcidid: 0000-0001-5104-5055 surname: Kumar fullname: Kumar, Randhir – sequence: 2 givenname: Dustin orcidid: 0000-0003-1255-1865 surname: Dzikonski fullname: Dzikonski, Dustin – sequence: 3 givenname: Elena surname: Bekker fullname: Bekker, Elena – sequence: 4 givenname: Robert orcidid: 0009-0004-0904-4824 surname: Vornhusen fullname: Vornhusen, Robert – sequence: 5 givenname: Valerio orcidid: 0000-0003-0965-7503 surname: Vitali fullname: Vitali, Valerio – sequence: 6 givenname: Jörg orcidid: 0000-0003-2222-9554 surname: Imbrock fullname: Imbrock, Jörg – sequence: 7 givenname: Cornelia orcidid: 0000-0002-7292-2499 surname: Denz fullname: Denz, Cornelia |
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| Cites_doi | 10.1088/2040-8978/13/4/044022 10.13070/mm.en.3.175 10.1038/s41598-019-49575-2 10.1016/j.addr.2012.09.010 10.1146/annurev.biophys.050708.133724 10.1002/ange.202110855 10.1039/C4RA06892K 10.1039/C8BM00474A 10.1063/1.3280222 10.1038/s41467-018-07473-7 10.1177/1535370217711441 10.1038/s41598-018-27456-4 10.1002/pen.760340213 10.1016/j.biomaterials.2003.08.021 10.1038/nrd3499 10.1002/bit.22361 10.1002/adma.201303680 10.1002/adma.200602649 10.1039/C8PY00278A 10.1038/s41598-021-93130-x 10.1016/j.micron.2007.06.011 10.1016/j.pmatsci.2017.08.003 10.1016/j.jphotochem.2006.03.004 10.1038/s41567-021-01368-z 10.1016/j.mattod.2016.02.020 10.1039/C7PY01202K 10.1002/adma.202106709 10.1016/j.actamat.2012.10.037 10.1122/1.550951 10.1038/s41578-020-0209-x 10.1073/pnas.1702208114 10.1021/la010075w 10.1038/s41598-020-62628-1 10.1039/b907992k 10.1088/1464-4258/9/8/S18 10.1002/adfm.201203880 10.1021/acs.biomac.5b01518 10.1016/j.addr.2011.06.009 10.3139/217.2360 10.1016/j.actbio.2017.07.028 10.1089/ten.tec.2019.0344 10.1021/acsnano.2c06104 10.1038/nmat3517 10.1021/ma970555n 10.1002/jbio.201600226 10.1021/cm5041584 10.1098/rsfs.2016.0011 10.1002/adhm.202001847 10.1016/j.actbio.2012.05.001 10.1016/j.biomaterials.2012.04.050 10.1007/s10544-017-0252-6 10.3390/mi8030065 10.1002/adma.200501612 10.1039/D0SM02168G 10.1039/C8SM00373D 10.1038/s41570-022-00404-7 10.1038/s41578-021-00350-8 10.1016/j.colsurfb.2005.01.010 |
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| References | Fischer (oe-31-18-29174-R45) 2007; 9 Tromayer (oe-31-18-29174-R62) 2018; 9 Antich (oe-31-18-29174-R2) 2021; 10 Nava (oe-31-18-29174-R42) 2018; 14 Revzin (oe-31-18-29174-R44) 2001; 17 Alberts (oe-31-18-29174-R31) 2015 Yang (oe-31-18-29174-R43) 2017; 8 Tibbitt (oe-31-18-29174-R22) 2009; 103 Hurst (oe-31-18-29174-R39) 2021; 17 Abele (oe-31-18-29174-R61) 2022; 34 Witkowska (oe-31-18-29174-R27) 2018; 8 Lih (oe-31-18-29174-R17) 2012; 8 Roberts (oe-31-18-29174-R49) 2007; 19 Liu (oe-31-18-29174-R54) 1998; 42 Kurokawa (oe-31-18-29174-R24) 2017; 114 Ciuciu (oe-31-18-29174-R33) 2014; 4 Kumar (oe-31-18-29174-R36) 2021; 11 McKinnon (oe-31-18-29174-R58) 2014; 26 Martino (oe-31-18-29174-R28) 2016; 6 Salehi-Reyhani (oe-31-18-29174-R10) 2017; 242 Knipe (oe-31-18-29174-R15) 2016; 17 Moeendarbary (oe-31-18-29174-R25) 2013; 12 Arora (oe-31-18-29174-R55) 2013; 3 Torgersen (oe-31-18-29174-R34) 2013; 23 Allen (oe-31-18-29174-R21) 2022; 6 Xu (oe-31-18-29174-R7) 2016; 19 Cox (oe-31-18-29174-R48) 1959 Tan (oe-31-18-29174-R3) 2004; 25 Fares (oe-31-18-29174-R14) 2018; 6 Tomatsu (oe-31-18-29174-R18) 2011; 63 Guindani (oe-31-18-29174-R6) 2022; 134 Kuznetsova (oe-31-18-29174-R60) 2007; 38 Jiang (oe-31-18-29174-R9) 2022; 16 Yoo (oe-31-18-29174-R8) 2011; 10 Ramos (oe-31-18-29174-R4) 2020; 26 Niederholtmeyer (oe-31-18-29174-R23) 2018; 9 Wirtz (oe-31-18-29174-R59) 2009; 38 Schnurr (oe-31-18-29174-R56) 1997; 30 Rodrigo-Navarro (oe-31-18-29174-R1) 2021; 6 Peppas (oe-31-18-29174-R16) 2006; 18 Billiet (oe-31-18-29174-R29) 2012; 33 Vitali (oe-31-18-29174-R47) 2020; 10 Gaharwar (oe-31-18-29174-R5) 2020; 5 Fernández-Pérez (oe-31-18-29174-R20) 2019; 9 Rodriguez (oe-31-18-29174-R26) 2005; 42 Hoffman (oe-31-18-29174-R13) 2012; 64 Jia (oe-31-18-29174-R50) 2015; 27 Wu (oe-31-18-29174-R32) 2006; 181 Ansari (oe-31-18-29174-R51) 2010; 25 Hasselmann (oe-31-18-29174-R35) 2018; 20 Vitali (oe-31-18-29174-R41) 2021; 17 Preece (oe-31-18-29174-R38) 2011; 13 Hörner (oe-31-18-29174-R40) 2017; 10 Bose (oe-31-18-29174-R30) 2018; 93 Kirschner (oe-31-18-29174-R12) 2013; 61 Shaw (oe-31-18-29174-R53) 1994; 34 Amaral (oe-31-18-29174-R57) 2018; 9 Vedadghavami (oe-31-18-29174-R11) 2017; 62 Yao (oe-31-18-29174-R37) 2009; 9 Fischer (oe-31-18-29174-R46) 2010; 81 |
| References_xml | – volume: 13 start-page: 044022 year: 2011 ident: oe-31-18-29174-R38 publication-title: J. Opt. doi: 10.1088/2040-8978/13/4/044022 contributor: fullname: Preece – volume: 3 start-page: 24 year: 2013 ident: oe-31-18-29174-R55 publication-title: Mater Methods doi: 10.13070/mm.en.3.175 contributor: fullname: Arora – volume: 9 start-page: 14933 year: 2019 ident: oe-31-18-29174-R20 publication-title: Sci. Rep. doi: 10.1038/s41598-019-49575-2 contributor: fullname: Fernández-Pérez – volume: 64 start-page: 18 year: 2012 ident: oe-31-18-29174-R13 publication-title: Adv. Drug Delivery Rev. doi: 10.1016/j.addr.2012.09.010 contributor: fullname: Hoffman – year: 1959 ident: oe-31-18-29174-R48 contributor: fullname: Cox – volume: 38 start-page: 301 year: 2009 ident: oe-31-18-29174-R59 publication-title: Annu. Rev. Biophys. doi: 10.1146/annurev.biophys.050708.133724 contributor: fullname: Wirtz – volume: 134 start-page: e202110855 year: 2022 ident: oe-31-18-29174-R6 publication-title: Angew. Chem. doi: 10.1002/ange.202110855 contributor: fullname: Guindani – volume: 4 start-page: 45504 year: 2014 ident: oe-31-18-29174-R33 publication-title: RSC Adv. doi: 10.1039/C4RA06892K contributor: fullname: Ciuciu – volume: 6 start-page: 2938 year: 2018 ident: oe-31-18-29174-R14 publication-title: Biomater. Sci. doi: 10.1039/C8BM00474A contributor: fullname: Fares – volume: 81 start-page: 015103 year: 2010 ident: oe-31-18-29174-R46 publication-title: Rev. Sci. Instrum. doi: 10.1063/1.3280222 contributor: fullname: Fischer – volume: 9 start-page: 5027 year: 2018 ident: oe-31-18-29174-R23 publication-title: Nat. Commun. doi: 10.1038/s41467-018-07473-7 contributor: fullname: Niederholtmeyer – volume: 242 start-page: 1309 year: 2017 ident: oe-31-18-29174-R10 publication-title: Exp. Biol. Med. doi: 10.1177/1535370217711441 contributor: fullname: Salehi-Reyhani – volume: 8 start-page: 9422 year: 2018 ident: oe-31-18-29174-R27 publication-title: Sci. Rep. doi: 10.1038/s41598-018-27456-4 contributor: fullname: Witkowska – volume: 34 start-page: 159 year: 1994 ident: oe-31-18-29174-R53 publication-title: Polym. Eng. Sci. doi: 10.1002/pen.760340213 contributor: fullname: Shaw – volume: 25 start-page: 1355 year: 2004 ident: oe-31-18-29174-R3 publication-title: Biomaterials doi: 10.1016/j.biomaterials.2003.08.021 contributor: fullname: Tan – volume: 10 start-page: 521 year: 2011 ident: oe-31-18-29174-R8 publication-title: Nat. Rev. Drug Discovery doi: 10.1038/nrd3499 contributor: fullname: Yoo – volume: 103 start-page: 655 year: 2009 ident: oe-31-18-29174-R22 publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.22361 contributor: fullname: Tibbitt – volume: 26 start-page: 865 year: 2014 ident: oe-31-18-29174-R58 publication-title: Adv. Mater. doi: 10.1002/adma.201303680 contributor: fullname: McKinnon – volume: 19 start-page: 2503 year: 2007 ident: oe-31-18-29174-R49 publication-title: Adv. Mater. doi: 10.1002/adma.200602649 contributor: fullname: Roberts – volume: 9 start-page: 3108 year: 2018 ident: oe-31-18-29174-R62 publication-title: Polym. Chem. doi: 10.1039/C8PY00278A contributor: fullname: Tromayer – volume: 11 start-page: 13917 year: 2021 ident: oe-31-18-29174-R36 publication-title: Sci. Rep. doi: 10.1038/s41598-021-93130-x contributor: fullname: Kumar – volume: 38 start-page: 824 year: 2007 ident: oe-31-18-29174-R60 publication-title: Micron doi: 10.1016/j.micron.2007.06.011 contributor: fullname: Kuznetsova – volume: 93 start-page: 45 year: 2018 ident: oe-31-18-29174-R30 publication-title: Prog. Mater. Sci. doi: 10.1016/j.pmatsci.2017.08.003 contributor: fullname: Bose – volume: 181 start-page: 1 year: 2006 ident: oe-31-18-29174-R32 publication-title: J. Photochem. Photobiol., A doi: 10.1016/j.jphotochem.2006.03.004 contributor: fullname: Wu – volume: 17 start-page: 1270 year: 2021 ident: oe-31-18-29174-R39 publication-title: Nat. Phys. doi: 10.1038/s41567-021-01368-z contributor: fullname: Hurst – volume: 19 start-page: 516 year: 2016 ident: oe-31-18-29174-R7 publication-title: Mater. Today doi: 10.1016/j.mattod.2016.02.020 contributor: fullname: Xu – volume: 9 start-page: 525 year: 2018 ident: oe-31-18-29174-R57 publication-title: Polym. Chem. doi: 10.1039/C7PY01202K contributor: fullname: Amaral – volume: 34 start-page: 2106709 year: 2022 ident: oe-31-18-29174-R61 publication-title: Adv. Mater. doi: 10.1002/adma.202106709 contributor: fullname: Abele – volume: 61 start-page: 931 year: 2013 ident: oe-31-18-29174-R12 publication-title: Acta Mater. doi: 10.1016/j.actamat.2012.10.037 contributor: fullname: Kirschner – volume: 42 start-page: 453 year: 1998 ident: oe-31-18-29174-R54 publication-title: J. Rheol. doi: 10.1122/1.550951 contributor: fullname: Liu – volume: 5 start-page: 686 year: 2020 ident: oe-31-18-29174-R5 publication-title: Nat. Rev. Mater. doi: 10.1038/s41578-020-0209-x contributor: fullname: Gaharwar – volume: 114 start-page: 7228 year: 2017 ident: oe-31-18-29174-R24 publication-title: Proc. Natl. Acad. Sci. doi: 10.1073/pnas.1702208114 contributor: fullname: Kurokawa – volume: 17 start-page: 5440 year: 2001 ident: oe-31-18-29174-R44 publication-title: Langmuir doi: 10.1021/la010075w contributor: fullname: Revzin – volume: 10 start-page: 5831 year: 2020 ident: oe-31-18-29174-R47 publication-title: Sci. Rep. doi: 10.1038/s41598-020-62628-1 contributor: fullname: Vitali – volume: 9 start-page: 2568 year: 2009 ident: oe-31-18-29174-R37 publication-title: Lab Chip doi: 10.1039/b907992k contributor: fullname: Yao – volume: 9 start-page: S239 year: 2007 ident: oe-31-18-29174-R45 publication-title: J. Opt. A: Pure Appl. Opt. doi: 10.1088/1464-4258/9/8/S18 contributor: fullname: Fischer – volume: 23 start-page: 4542 year: 2013 ident: oe-31-18-29174-R34 publication-title: Adv. Funct. Mater. doi: 10.1002/adfm.201203880 contributor: fullname: Torgersen – volume: 17 start-page: 788 year: 2016 ident: oe-31-18-29174-R15 publication-title: Biomacromolecules doi: 10.1021/acs.biomac.5b01518 contributor: fullname: Knipe – volume: 63 start-page: 1257 year: 2011 ident: oe-31-18-29174-R18 publication-title: Adv. Drug Delivery Rev. doi: 10.1016/j.addr.2011.06.009 contributor: fullname: Tomatsu – volume: 25 start-page: 287 year: 2010 ident: oe-31-18-29174-R51 publication-title: International Polymer Processing doi: 10.3139/217.2360 contributor: fullname: Ansari – volume: 62 start-page: 42 year: 2017 ident: oe-31-18-29174-R11 publication-title: Acta Biomater. doi: 10.1016/j.actbio.2017.07.028 contributor: fullname: Vedadghavami – volume: 26 start-page: 91 year: 2020 ident: oe-31-18-29174-R4 publication-title: Tissue Eng., Part C doi: 10.1089/ten.tec.2019.0344 contributor: fullname: Ramos – volume: 16 start-page: 15705 year: 2022 ident: oe-31-18-29174-R9 publication-title: ACS Nano doi: 10.1021/acsnano.2c06104 contributor: fullname: Jiang – volume: 12 start-page: 253 year: 2013 ident: oe-31-18-29174-R25 publication-title: Nat. Mater. doi: 10.1038/nmat3517 contributor: fullname: Moeendarbary – year: 2015 ident: oe-31-18-29174-R31 contributor: fullname: Alberts – volume: 30 start-page: 7781 year: 1997 ident: oe-31-18-29174-R56 publication-title: Macromolecules doi: 10.1021/ma970555n contributor: fullname: Schnurr – volume: 10 start-page: 1492 year: 2017 ident: oe-31-18-29174-R40 publication-title: J. Biophotonics doi: 10.1002/jbio.201600226 contributor: fullname: Hörner – volume: 27 start-page: 387 year: 2015 ident: oe-31-18-29174-R50 publication-title: Chem. Mater. doi: 10.1021/cm5041584 contributor: fullname: Jia – volume: 6 start-page: 20160011 year: 2016 ident: oe-31-18-29174-R28 publication-title: Interface Focus doi: 10.1098/rsfs.2016.0011 contributor: fullname: Martino – volume: 10 start-page: 2001847 year: 2021 ident: oe-31-18-29174-R2 publication-title: Adv. Healthcare Mater. doi: 10.1002/adhm.202001847 contributor: fullname: Antich – volume: 8 start-page: 3261 year: 2012 ident: oe-31-18-29174-R17 publication-title: Acta Biomater. doi: 10.1016/j.actbio.2012.05.001 contributor: fullname: Lih – volume: 33 start-page: 6020 year: 2012 ident: oe-31-18-29174-R29 publication-title: Biomaterials doi: 10.1016/j.biomaterials.2012.04.050 contributor: fullname: Billiet – volume: 20 start-page: 8 year: 2018 ident: oe-31-18-29174-R35 publication-title: Biomed. Microdevices doi: 10.1007/s10544-017-0252-6 contributor: fullname: Hasselmann – volume: 8 start-page: 65 year: 2017 ident: oe-31-18-29174-R43 publication-title: Micromachines doi: 10.3390/mi8030065 contributor: fullname: Yang – volume: 18 start-page: 1345 year: 2006 ident: oe-31-18-29174-R16 publication-title: Adv. Mater. doi: 10.1002/adma.200501612 contributor: fullname: Peppas – volume: 17 start-page: 3105 year: 2021 ident: oe-31-18-29174-R41 publication-title: Soft Matter doi: 10.1039/D0SM02168G contributor: fullname: Vitali – volume: 14 start-page: 3288 year: 2018 ident: oe-31-18-29174-R42 publication-title: Soft Matter doi: 10.1039/C8SM00373D contributor: fullname: Nava – volume: 6 start-page: 562 year: 2022 ident: oe-31-18-29174-R21 publication-title: Nat. Rev. Chem. doi: 10.1038/s41570-022-00404-7 contributor: fullname: Allen – volume: 6 start-page: 1175 year: 2021 ident: oe-31-18-29174-R1 publication-title: Nat. Rev. Mater. doi: 10.1038/s41578-021-00350-8 contributor: fullname: Rodrigo-Navarro – volume: 42 start-page: 125 year: 2005 ident: oe-31-18-29174-R26 publication-title: Colloids Surf., B doi: 10.1016/j.colsurfb.2005.01.010 contributor: fullname: Rodriguez |
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