The GDI1 genes from Kluyveromyces lactis and Pichia pastoris: cloning and functional expression in Saccharomyces cerevisiae

• Published in: Yeast (Chichester, England) Vol. 18; no. 10; pp. 897 - 902
• Main Authors: Brummer, Mirko H., Richard, Peter, Sundqvist, Lena, Väänänen, Ritva, Keränen, Sirkka
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-07-2001
• Subjects: Amino Acid Sequence; Base Sequence; Cloning, Molecular; GDI1; GDI1 gene; Genetic Complementation Test; Guanine Nucleotide Dissociation Inhibitors - biosynthesis; Guanine Nucleotide Dissociation Inhibitors - genetics; Kluyveromyces - genetics; Kluyveromyces lactis; Molecular Sequence Data; Pichia - genetics; Pichia pastoris; Recombinant Proteins - biosynthesis; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; secretion; Sequence Homology, Amino Acid
• ISSN: 0749-503X; 1097-0061
• DOI: 10.1002/yea.736

The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp (K.lactis) and 1344 bp (P. pastoris), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 (K.lactis GDI1) and AY007574 (P. pastoris GDI1). Copyright © 2001 John Wiley & Sons, Ltd.