Evaluation of silver nanoparticle-impregnated PMMA loaded with vancomycin or gentamicin against bacterial biofilm formation

• Published in: Injury Vol. 54 Suppl 6; p. 110649
• Main Authors: Soni, Jamil Faissal, Ribeiro, Victoria Stadler Tasca, Cieslinski, Juliette, de Andrade, Ana Paula, Dantas, Letícia Ramos, Pereira, Bruna Zanatta, de Almeida, Beatriz Martins Rodrigues Carvalho, Suss, Paula Hansen, Tuon, Felipe Francisco
• Format: Journal Article
• Language: English
• Published: Netherlands 01-11-2023
• Subjects: Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Bacteria; Biofilms; Bone Cements - pharmacology; Escherichia coli; Gentamicins - pharmacology; Humans; Metal Nanoparticles; Polymethyl Methacrylate - pharmacology; Silver - pharmacology; Staphylococcus aureus; Vancomycin - pharmacology; Antimicrobial; Infection; Nanoparticles; Mechanical properties; Bone cement
• ISSN: 0020-1383; 1879-0267
• DOI: 10.1016/j.injury.2023.02.032

Bone cement containing vancomycin or gentamicin is a therapeutic strategy for combating orthopedic infections: however, the activity of these antibiotics is narrow. Silver nanoparticles (AgNPs) are nanocomponents with a wide spectrum, including multidrug-resistant bacteria. In the present study, we aimed to evaluate the effect of AgNP-loaded polymethylmethacrylate (PMMA) on biofilm formation by Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus epidermidis.
The effect of AgNP-loaded PMMA with and without vancomycin or gentamicin on biofilm production was quantitatively analyzed. S. aureus, E. coli, P. aeruginosa, and S. epidermidis were included as biofilm-producing microorganisms in the in vitro model.
AgNP-loaded PMMA with antibiotics reduced the number of colony-forming units (CFUs; p<0.001). However, AgNP-loaded PMMA alone did not significantly reduce biofilm formation.
Our study demonstrated the potential of AgNP-loaded PMMA. Notably, we observed that AgNP-loaded PMMA containing vancomycin or gentamycin exhibited significantly superior efficacy, with satisfactory activity against most biofilm-forming microbial agents examined.